| 摘要: |
| 以黄口荔枝螺转录组测序所得到的拼接序列为基础, 利用 MISA 软件进行微卫星分析, 利用Primer5.0 设计引物 160 对。结果显示, 83 对引物能够成功扩增, 引物在黄口荔枝螺渔山列岛群体多态性检测中发现, 37 个 SSR 位点能够表现出多态性, 一共获得了 129 个等位基因, 各位点等位基因数为2～5 个, 平均每个位点等位基因数为 3.49 个, 观测杂合度 Ho, 期望杂合度He, 多态信息含量PIC范围分别介于 0.000～0.800、0.146～0.645、0.139～0.573 之间, 有 9 个表现为高度多态, 20 个表现为中度多态,8 个表现为低度多态。实验结果进行 Hardy-Weinberg 平衡检测后, 利用 Bonferroni correction 法进行校正, 有 29 个位点显著偏离, 结果表明渔山列岛黄口荔枝螺群体总体遗传多样性水平较低。 |
| 关键词: 黄口荔枝螺 转录组 微卫星 遗传多样性 |
| DOI：10.11759/hykx20150616002 |
| 分类号: |
| 基金项目:宁波市农业科技攻关项目(2014C10018) |
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| Characterization and analysis of microsatellite markers in Thais luteostoma using next generation sequencing |
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| Abstract: |
| Thais luteostoma is one of the most important marine shellfish species, which widely inhabits throughout the southern and northern coastal areas in China. In this paper, we developed a series of microsatellite markers using a transcriptome-based sequencing to provide a foundation for genetic research on Thais luteostoma. We sequenced a large number of ESTs and screened 160 potential microsatellites of nucleotide repeats using MISA software. We successfully obtained clear reproducible bands for 83 microsatellite loci. These were amplified in 30 wild individuals sampled from Yushan Island, Ningbo, Zhejiang province. 37 SSR loci were polymorphic (number of alleles per locus ranged from 2 to 5, with an average of 3.49). The observed heterozygosity ranged from 0.000 to 0.800, while the expected heterozygosity varied from 0.146 to 0.645. Polymorphic information content ranged from 0.139 to 0.573, and 29 SSR loci deviated from Hardy-Weinberg equilibrium. Among these, 9 loci had a PIC of <0.5, so were classified as highly polymorphic, additional 20 loci were moderately polymorphic with PICs ranging from 0.25 to 0.5. The PIC of 8 loci was <0.25, indicating that these were classified as low polymorphic loci. The results show that the genetic diversity of T. luteostoma is low in Yushan Island. |
| Key words: Thais luteostoma transcriptome sequencing microsatellite markers polymorphism |
