引用本文:	韦国建,刘文广,林坚士,何毛贤.马氏珠母贝两个与生长性状相关QTL 的验证[J].海洋科学,2015,39(11):13-19.

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马氏珠母贝两个与生长性状相关QTL 的验证
韦国建^1,2, 刘文广¹, 林坚士¹, 何毛贤¹
1.中国科学院热带海洋生物资源与生态重点实验室, 广东省应用海洋生物学重点实验室, 中国科学院南海海洋研究所;2.中国科学院大学

摘要:

利用2 个马氏珠母贝群体对遗传连锁图谱已经定位的, 与马氏珠母贝生长性状相关的数量性状位点(Quantitative trait locus, QTL)中的两个进行验证。结果显示: m59 标记在2 个群体中与壳高、壳长、壳宽和总重具有显著的相关性(P<0.05), 其GT 基因型的个体壳高、壳长、壳宽和总重均显著高于GG基因型的个体; 154165 标记在湛江群体中与壳高具有显著相关性(P<0.05), 而在深圳群体中与壳宽具有显著相关性(P<0.05); 表明QTL 标记m59 在2 个群体中是共享QTL, 而154165 标记在群体中是特异QTL。基于这两个QTL 在这2 个群体中分别构建二倍型, 发现在湛江群体中二倍型C₁(AAGT)和深圳群体中D₁(AGGT)型和D₂(AAGT)型对马氏珠母贝生长最为有利, 可作为优先的基因型组合。

关键词: 马氏珠母贝数量性状位点(Quantitative trait locus, QTL) 验证生长性状

DOI：10.11759/hykx20150731004

分类号:

基金项目:国家863 计划项目(2012AA10A410); 广东省科技计划项目(2013B020308005); 广东省海洋渔业科技推广专项(A201301A03)

Verification of two QTL associated with growth traits of Pear oyster Pinctada martensii Dunker

Abstract:

Quantitative trait loci (QTL) affecting growth traits have been mapped in genetic map of Pinctada martensii. In this study, two of these QTL were verified in two oysters different genetic backgrounds. The results showed that the locus m59 was significantly associated with shell height, shell length, shell width and total weight in these two populations (P<0.05), and oysters with genotypes GT grew faster than those with the genotype GG in shell height, shell length, shell width and total weight. QTL154165 was significantly associated with shell height in Zhanjiang population (P<0.05), but it was significantly associated with shell width in Shenzhen population (P<0.05). Diplotypes were constructed based on the two QTLs in Zhanjiang population and Shenzhen population, and association analyses revealed that C₁ (AAGT) in Zhanjiang population and D₁ (AGGT) and D₂ (AAGT) in shenzhen population might be the most advantageous diplotypes for growth traits.

Key words: Pinctada martensii Quantitative trait locus (QTL) Verification Growth trait