引用本文:	王佳宁,李晓明,张鹏,徐刚明,王斌贵.日本仙菜来源内生真菌Aspergillus versicolorEN-298化学成分研究[J].海洋科学,2015,39(6):94-98.

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*日本仙菜来源内生真菌Aspergillus versicolorEN-298化学成分研究*
王佳宁^1,2, 李晓明¹, 张鹏^1,2, 徐刚明¹, 王斌贵¹
1.中国科学院海洋研究所实验海洋生物学重点实验室;2.中国科学院大学

摘要:

从采自烟台养马岛的日本仙菜(Ceramium japonicum)中分离得到的一株内生真菌Aspergillus versicolorEN-298, 对其次级代谢产物进行了系统研究, 利用反相柱层析、正相柱层析、葡聚糖凝胶Sephadex LH-20 以及制备薄层层析(pTLC)等分离手段获得10 个单体化合物。通过一维、二维核磁共振技术(1D、2D NMR)等鉴定了所有化合物的结构。并对分离得到的化合物进行了抑菌活性测试, 其中化合物10 对迟缓爱德华氏菌(Edwardsiella tarda)表现出一定的抑制作用。

关键词: 日本仙菜(Ceramium japonicum) 内生真菌化学成分结构鉴定

DOI：10.11759/hykx20150112002

分类号:

基金项目:国家自然科学基金项目(31330009)

Chemical constituents of Aspergillus versicolorEN-298, an endophytic fungus derived from the marine alga Ceramium japonicum

Abstract:

Ten compounds were isolated from the secondary metabolites of Aspergillus versicolor EN-298, an endophytic fungus that was isolated from the marine red alga Ceramium japonicum. The structures of the isolated compounds were elucidated by means of spectroscopic methods including UV and NMR (1D and 2D) as well as by comparison with authentic standards and literature reports. All compounds were evaluated for antimicrobial activity, and the compound 10 showed moderate activity against aqua-bacterial Edwardsiella tarda with an MIC value of 16 μg/mL.

Key words: Ceramium japonicum Endophytic fungus chemical constituents structure elucidation