| 摘要: |
| 为了了解pH 胁迫下脊尾白虾(Palaemon(Exopalaemon) carinicauda Holthuis )的适应性, 作者采用静水毒性的实验方法确定了脊尾白虾的半致死pH, 分析了pH 胁迫对脊尾白虾抗氧化酶活力的影响。设置pH4～11.1 共17 个胁迫组, 统计脊尾白虾24、48、72 和96 h 的死亡率。结果显示, 脊尾白虾24、48、72 和96 h 酸性半致死pH 值分别为4.25、4.64、4.71 和4.78, 碱性半致死pH 分别为10.87、10.55、10.38 和10.29。将脊尾白虾暴露于低pH 6.5、高pH9.5 的水体, 以pH8.0 作为对照, 分别于胁迫后0、3、6、12、24、48、72 和96 h 测定鳃、肝胰腺、肌肉和血淋巴中总抗氧化力、过氧化氢酶、抗超氧阴离子活力。结果发现, pH 胁迫后, 在3～6 h 内各组织中抗氧化酶活力均显著上升, 24～48 h 开始回落, 到96 h 之前, 各组织中抗氧化酶水平基本恢复到对照组水平。整个实验过程中肝胰腺中抗氧化酶活力水平明显高于其他组织。本实验结果表明, 脊尾白虾对pH 有很强的适应性, pH 胁迫3～24 h 之内抗氧化酶活力反馈性升高清除体内多余的活性氧。 |
| 关键词: 脊尾白虾(Palaemon(Exopalaemon) carinicauda Holthuis) 半致死pH pH 胁迫 抗氧化酶 |
| DOI：10.11759/hykx20140418001 |
| 分类号: |
| 基金项目:主要养殖甲壳类良种培育课题(2012AA10A409); 国家虾产业技术体系(CARS-47); 山东省自主创新专项(2013CX80202); 公益性行业(农业)科研专项经费项目(201103034); 南通市科技项目(HL2013010) |
|
| The effects of pH changes on antioxidant enzyme activities of ridgetail white prawn (Palaemon carinicauda) |
|
|
| Abstract: |
| The ridgetail white prawn Palaemon carinicauda is an important mariculture species in China. In this study, the adaptability of P. carinicauda to pH stress was investigated. The median lethal pH was measured in the hydrostatic toxicity experiment and the effect of pH changes on the antioxidant enzyme activities of ridgetail white prawn was studied. The death rates were calculated under different pH stress from pH 4.0 to pH 11.1 for 24, 48, 72 or 96 h. The median lethal pH in the acidic range was 4.25 for 24 h, 4.64 for 48 h, 4.71 for 72 h and 4.78 for 96 h, respectively. The LpH50 in the alkaline zone was 10.87 for 24 h, 10.55 for 48 h, 10.38 for 72 h and 10.29 for 96 h, respectively. The ridgetail white prawns were exposed to water of pH 6.5 or pH 9.5 for 96 h with pH 8 set as contrast. Total antioxidation activity, catalase activity and anti-superoxide anion activity were determined in gill, hepatopancreas, muscle and haemocytes after 0, 3, 6, 12, 24, 48, 72 and 96 h exposure. The activities of T-AOC and CAT and the anti-superoxide anion activity in different tissues significantly increased within 3～6 h of pH stress. The antioxidant enzyme activities decreased from 24 to 48 h, and then fell back to levels of control groups basically before 96 h. The antioxidant enzyme activities in hepatopancreas were highest under pH stress. The results indicated that ridgetail white prawn was highly adaptable to pH stress. The antioxidant enzyme activities would increase for feedback to eliminate unnecessary reactive oxygen within 3～24 h. |
| Key words: Palaemon carinicauda LpH50 pH stress antioxidant enzyme |
