| 摘要: |
| 以水解度为指标, 比较了6 种蛋白酶对香港巨牡蛎 (Crassostrea hongkongensis) 的水解效果。在单因素考察实验的基础上, 采用 Box-Behnken 模型响应面设计, 建立香港巨牡蛎蛋白酶解的二次回归模型, 确定最佳的水解条件。实验结果表明, 海产品专用蛋白酶对香港巨牡蛎蛋白的水解效果优于其他蛋白酶, 该酶的最佳水解条件为: 酶与底物比为 5.93 %, 酶解时间为 4.07 h, 酶解温度为 54.6 ℃。在此条件下, 海产品专用蛋白酶酶解香港巨牡蛎蛋白的水解度达到 25.73 % ± 2.39 %。本研究为香港巨牡蛎的开发和利用提供了理论依据。 |
| 关键词: 香港巨牡蛎(Crassostrea hongkongensis) 水解度 响应面分析 |
| DOI：10.11759/hykx20140609001 |
| 分类号: |
| 基金项目:广西科学院基本科研业务费资助项目(13YJ22HYS15); 广西科学研究与技术开发计划项目(桂科攻 09321004) |
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| Improvement of Crassostrea hongkongensis hydrolysis by Response Surface Methodology |
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| Abstract: |
| Using DH (the degree of hydrolysis) as the main index, the hydrolysis of Crassostrea hongkongensis using six proteases was compared. On the basis of single factor experiment, response surface methodology and Box-Behnken model were employed to establish the quadratic model for optimization of the hydrolysis conditions. Results showed that the DH of marine products protein-specific protease was higher than that of others. The optimum hydrolysis condition was as follows: the ratio of enzyme to substrate was 5.93%, hydrolysis time was 4.07 h and the hydrolysis temperature was 54.6℃. Under this condition, DH reached 25.73%±2.39 %. This study aims to provide theoretical basis for the development and utilization of C. hongkongensis. |
| Key words: Crassostrea hongkongensis Degress of hydrolysis Response surface methodology (RSM) |
