| 摘要: |
| 为检测中国近海海洋环境中菊酯类农药等污染物对日本囊对虾(Marsupenaeus japonicas)的基因毒性及其危害程度, 作者首次使用醚菊酯对日本囊对虾进行毒理实验, 将日本囊对虾分为6 组进行不同剂量的处理, 采用彗星实验分析技术对处理培育25 d 的日本囊对虾DNA损伤情况进行检测, 并通过Comet Score 1.5 分析软件对实验图谱进行拖尾率、尾长、彗尾DNA 相对含量、尾距、Olive 距等DNA损伤指标的统计分析, 在对各指标分别建立一元回归方程的基础上, 又通过SPSS 软件进行判定分析,筛选影响作用更大的检测指标, 建立了多参数的多元回归方程。实验结果发现, 与空白对照组相比, 日本囊对虾各实验处理组肝胰腺细胞DNA 均有不同程度的损伤且各检测指标均有显著性差异(P<0.01),各检测指标与处理浓度表现出良好的剂量效应关系, 随着处理浓度递增, 各检测指标都呈规律性的增长趋势。回归分析和相关系数表明: 各检测指标与处理浓度具有高度的相关性, 多项式回归方程(P<0.05)和多元回归方程(P<0.005)均有显著性的统计学意义, 通过多元回归方程可以有效地推断醚菊酯处理浓度和处理时间。 |
| 关键词: 日本囊对虾(Marsupenaeus japonicas) 醚菊酯 DNA 损伤 彗星实验 |
| DOI：10.11759/hykx20131122002 |
| 分类号: |
| 基金项目:国家海洋局海洋公益性行业科研专项(201005012) |
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| The research on DNA damage in hepatopancreas cells of Marsupenaeus japonicas exposed to ethofenprox |
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| Abstract: |
| In order to analyze the genotoxicity of Marsupenaeus japonicas caused by the pollutant of pyrethroid pesticides in the coastal sea of China, here, we applied ethofenprox in toxicological experiment on M. japonicas for the first time. In our study, M. japonicas were divided into six groups and exposed to different concentrations of ethofenprox for 25 days. DNA damage in hepatopancreas cells of M. japonicas was evaluated with comet assay. The DNA damage parameters such as the comet rate, the tail length, the percentage of tail DNA, the tail moment and the olive moment were statistically analyzed by Comet Score 1.5. On the basis of one-dimensional regression equation, we selected greater influenced parameters after comparing with SPSS 16.0 and constructed the multiple-dimensional regression equation. The results show that DNA damage in hepatopancreas cells was observed in all exposed groups and the parameters of each exposed group were significantly different from the control group (P<0.01). There was a dose-effect relationship between ethofenprox and DNA damage in hepatopancreas cells, which increased regularly with the exposure concentrations. The regression analysis and coefficient indicated that all DNA damage parameters were highly relevant to the exposed concentrations. The one-dimensional regression equation (P<0.05) and multiple-dimensional regression equation (P<0.005) had significant statistic meaning, which might be an effective way to estimate the exposed concentration and time of ethofenprox. |
| Key words: Marsupenaeus japonicas ethofenprox DNA damage comet assay |
