| 引用本文: | 肖永双,肖志忠,刘清华,滕照军,徐世宏,马道远,李 军.星斑川鲽、大菱鲆及其杂交后代的线粒体DNA 序列比较分析[J].海洋科学,2014,38(6):5-9. |
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| 摘要: |
| 为了揭示星斑川鲽(Platichthys stellatus)♀×大菱鲆(Scophthalmus maximus)♂杂交后代的种质遗传属性, 作者采用线粒体(mt)DNA COI 基因片段和控制区(D-loop)序列对星斑川鲽、大菱鲆及两种间的杂交后代(星斑川鲽♀×大菱鲆♂)遗传特性进行研究。研究结果显示, 基于mtDNA COI 和D-loop 序列结果显示星斑川鲽和大菱鲆的种间遗传距离分别为22.9%和41.2%, 相似的研究结果显示星斑川鲽♀×大菱鲆♂杂交后代与大菱鲆的遗传距离分别为22.9%和41.2%, 遗传差异极其显著。而基于mtDNA D-loop序列结果显示星斑川鲽♀×大菱鲆♂杂交后代与星斑川鲽的遗传距离仅为0.4%, 并且在mtDNA COI 基因片段上两者序列完全一致, 杂交后代在线粒体DNA 上呈现明显的偏母遗传。 |
| 关键词: 星斑川鲽(Platichthys stellatus) 大菱鲆(Scophthalmus maximus) 杂交 线粒体DNA |
| DOI：10.11759/hykx20130903001 |
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| 基金项目:鲆鲽类现代产业技术体系建设项目(Nycytx-50-03); 山东省科技发展计划 (2011GGF01035; 2011GHY11516);山东省自主创新成果转化重大专项(2012ZHZX1C0802);青岛市科技发展计划项目(12-4-1-51-hy; 12-1-4-8-(6)-jch); 江苏省自然基金青年基金资助项目(BK2012222) |
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| Comparative analysis of genetic variability of mtDNA sequence in Platichthys stellatus, Scophthalmus maximus and their hybrids |
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| Abstract: |
| Partial COI gene fragments and D-loop sequences of mitochondrial DNA were used to estimate genetic variability in Platichthys stellatus, Scophthalmus maximus and their hybrids (P. stellatus♀×S. maximus♂). Based on the analyses of the mtDNA COI gene and D-loop fragments, the genetic distances (D) between P. stellatus and S. maximus were 22.9% and 41.2% respectively. Similar result was obtained between the hybrids (P. stellatus♀×S. maximus♂) and S. maximus, which showed significant differentiation between hybrids and male parent. A very low differentiation (D = 0.4%) was observed between the hybrid and female parent based on the mtDNA D-loop sequences, and no genetic differentiation was found between them based on the mtDNA COI gene sequences. According to the above results, the hybrids (P. stellatus♀×S. maximus♂) showed strict maternal inheritance in mtDNA genome. |
| Key words: Platichthys stellatus Scophthalmus maximus hybrids mtDNA |
