| 摘要: |
| 三氯异氰尿酸(Trichloroisocyanuric acid ,TCCA)是水产养殖中常用的水质消毒剂, 使用不当会给生产和环境带来危害。为指导脊尾白虾(Exopalaemon carinicauda)人工育苗过程中TCCA 的安全用药,作者利用静水试验法研究了TCCA 对脊尾白虾幼体的急性毒性及对幼体发育的影响。实验设7 个浓度梯度组(1.0、1.5、2.0、2.5、3.0、3.5、4.0 mg/L)和1 个空白对照组, 每组3 个平行。急性毒性实验持续96 h 后结束, 用正常育苗海水代替TCCA 溶液, 正常管理直到幼体全部变态为仔虾, 评估短时间的TCCA 处理对脊尾白虾幼体发育的影响。结果表明, TCCA 对脊尾白虾幼体在24、48、72 和96 h 的半致死浓度分别为3.81、3.03、2.71 和2.47 mg/L, 其安全浓度为0.25 mg/L; 随着TCCA 浓度的升高, 脊尾白虾幼体变态存活率显著降低(P<0.05), 但能成功变态为仔虾个体的变态持续时间及仔虾体长与对照组无明显差异(P>0.05)。本实验结果可以为脊尾白虾人工育苗安全用药提供参考和依据。 |
| 关键词: 脊尾白虾(Exopalaemon carinicauda) 三氯异氰尿酸 幼体发育 半致死浓度 安全浓度 |
| DOI： |
| 分类号: |
| 基金项目:国家科技支撑计划项目(2011BAD13B01); 国家自然科学基金面上项目(41376165); 公益性行业(农业)科研专项经费项目(201103034) |
|
| Effects of trichloroisocyanuric acid on the survival and development of Exopalaemon carinicauda larvae |
|
|
| Abstract: |
| Trichloroisocyanuric acid (TCCA) is a common disinfectant used in aquaculture, however, improper use of TCCA will bring hazard both to aquaculture and environment. In order to learn the effect of TCCA on aquaculture, a static bioassay test was conducted to investigate the acute toxicity of TCCA and its effects on the larvae development of Exopalaemon carinicauda. New-hatched larvae of E. carinicauda were exposed to eight concentrations of TCCA (0, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 mg/L for 96 h, and three replicates was set under each concentration level. After each exposure of TCCA, the survived individuals were reared under normal conditions till developed to the postlarvae stage. The metamorphosis survival, metamorphosis duration and body length of the postlarvae were compared to evaluate the possible chronic toxicity of TCCA to E. carinicauda larvae. The results showed that the 24, 48, 72 and 96 h LC50 are 3.81, 3.03, 2.71 and 2.47 mg/L, respectively. The safe concentration (SC) of TCCA to E. carinicauda larvae is 0.25 mg/L. The metamorphosis survival decreased significantly (P<0.05) with the increases of TCCA concentration, while there was no significant difference in metamorphosis duration and body length of the postlarvae for the survived individuals among different TCCA concentrations (P>0.05). The results in the present study provide important references for safe medication in nursery of E. carinicauda. |
| Key words: Exopalaemon carinicauda trichloroisocyanuric acid larvae development median lethal concentra-tion safe concentration |
