| 引用本文: | 刘 博,邵艳卿,王 侃,滕爽爽,柴雪良,方 军,张炯明,肖国强.4个缢蛏群体遗传多样性和系统发生关系的微卫星分析[J].海洋科学,2013,37(8):96-102. |
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| 摘要: |
| 采用12个微卫星分子标记,对4个不同缢蛏(Sinonovacula Constricta)群体: 福建云霄群体、广东湛江群体、天津塘沽群体以及浙江乐清湾群体进行了遗传多样性和系统发生关系的分析。研究结果显示, 12个微卫星共有46个等位基因, 4个缢蛏群体的平均观测等位基因数(Ao)、平均观测杂合度(Ho)、平均期望杂合度(He)和平均多态信息含量(PIC)分别为3.25～3.50、0.59～0.74、0.55～0.58 和0.46～0.50。说明4个缢蛏群体遗传多样性处于中等多态。UPGMA聚类分析表明:乐清湾群体、天津塘沽群体间的遗传距离最小, 亲缘关系最近, 首先聚为一支, 福建云霄群体和广东湛江群体聚为另一支。群体的F-统计量(Fst)为0.07, 表明缢蛏群体分化很微弱。 |
| 关键词: 缢蛏(Sinonovacula Constricta) 微卫星 遗传多样性 遗传距离 |
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| 基金项目:浙江省海洋水产养殖重点创新团队专项项目(2010R50025);浙江省重大科技专项项目(2012C12907-4); 国家贝类产业技术体系项目(CARS-48) |
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| Microsatellite analysis of genetic diversity and phylogenetic relationship of four different geographical populations of Sinonovacula Constricta |
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| Abstract: |
| Twelve microsatellite markers were used to study genetic diversity and phylogenetic relationship of four geographic populations of razor clam Sinonovacula Constricta. The four populations were sampled from Yunxiao City of Fujian, Zhanjiang City of Guangdong, Tanggu City of Tianjin and Yueqing City of Zhejiang. The results showed that total of 46 alleles were found among 12 SSR markers. The average observed number of alleles (o) was from 3.25 to 3.50, the average observed heterozygosity (Ho) was from 0.59 to 0.74, the average excepted heterozygosity (He) was from 0.55 to 0.58 and the average polymorphism information content (PIC) ranged from 0.46 to 0.50, respectively. All of these results indicated that genetic diversity of the four populations was at a medium level. UPGMA cluster analysis showed that Yueqing population was clustered with Tanggu population into one clad, then Yunxiao population and Zhanjiang population were in a second group. The value of the F-statistics was 0.07 and this suggested that the four populations of S．Constricta had a relatively lower genetic variation and its genetic structure between populations was weak. |
| Key words: Sinonovacula Constricta microsatellite genetic diversity genetic distance |
