引用本文:	张立田,戴习林,臧维玲.Ca²⁺、Mg²⁺及盐度对凡纳滨对虾体内代谢酶的影响[J].海洋科学,2013,37(7):63-71.

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Ca²⁺、Mg²⁺及盐度对凡纳滨对虾体内代谢酶的影响
张立田^1,2, 戴习林¹, 臧维玲¹
1.上海海洋大学水产与生命学院;2.东海水产研究所

摘要:

为探讨Ca²⁺、Mg²⁺、盐度对凡纳滨对虾体内代谢酶的相对独立作用和相互影响, 进而为提高凡纳滨对虾生长力和免疫力提供理论依据, 本实验采取L49(7⁸)安排7 水平Ca²⁺、Mg²⁺、盐度, L8(2⁷)安排2 水平Ca²⁺、Mg²⁺、盐度, 开展60 d 凡纳滨对虾(Litopenaeus vannamei)养殖试验, 通过比较对虾体内消化酶、ATP 酶及免疫类酶的活性以分析Ca²⁺、Mg²⁺、盐度对凡纳滨对虾生长力和免疫力的影响。结果表明: 水体中Ca²⁺、Mg²⁺、盐度对消化酶具有显著影响(P<0.05), 其中与对虾消化吸收联系最紧密的蛋白酶中, 盐度对胃蛋白酶影响显著, 盐度为10 时酶活最高, Ca²⁺、盐度对类胰蛋白酶影响显著, Ca²⁺为200 mg/L, 盐度为20 时, 酶活最高; Ca²⁺、Mg²⁺、盐度对ATP 酶具有显著影响(P<0.05), 其中对Na⁺-K⁺-ATP 酶都有显著影响, Ca²⁺为300 mg/L, Mg²⁺为500 mg/L, 盐度为30 时酶活最高, Ca²⁺、Mg²⁺对Mg²⁺-ATP 酶具有显著影响, Ca²⁺为200 mg/L, Mg²⁺为500 mg/L 时酶活最高, Ca²⁺对Ca²⁺-ATP 酶具有显著影响, Ca²⁺为200、300 mg/L 时酶活最高; Ca²⁺、Mg²⁺、盐度对凡纳滨对虾体内免疫酶具有显著影响(P<0.05), Ca²⁺、Mg²⁺、盐度对ACP 都有显著影响, Ca²⁺为100 mg/L, Mg²⁺为150 mg/L, 盐度为30 时酶活最高, Mg²⁺对AKP 具有显著影响, 在150 mg/L 时酶活最高, Ca²⁺、盐度对SOD 酶活具有显著影响,Ca²⁺为100 mg/L, 盐度为35 时酶活最高; Ca²⁺、Mg²⁺、盐度间的交互作用对体内代谢酶也有一定影响。

关键词: 凡纳滨对虾(Litopenaeus vannamei) Ca²⁺ Mg²⁺ 盐度消化酶 ATP 酶免疫类酶

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基金项目:上海市科技兴农重点攻关项目(沪农科攻字(2010)第1-6 号);上海市科技兴农科技推广项目(沪农科推字(2008)第5-1 号); 上海市教育委员会重点学科建设项目(J50701)

Effect of Ca²⁺, Mg²⁺ and salinity on metabolic enzymes of Litopenaeus vannamei

Abstract:

The L49(7⁸) orthogonal experimental design was used to investigate the three factors Ca²⁺, Mg²⁺ and salinity in water, and seven levels of each factor were based. The L49(7⁸) orthogonal experimental design was used to investigate the three factors Ca²⁺, Mg²⁺ and salinity in water, and two levels of each factor were based. In order to analyze the effect of Ca²⁺, Mg²⁺ and salinity in water on the growth performance and immunity of Litopenaeus vannamei, the digestive enzymes, ATP enzyme and immune enzymes were compared during the 60 d culture cycle. Results showed that Ca²⁺, and salinity had significant effect on digestive enzymes(P<0.05), the salinity had significant effect on pepsin, and the Ca²⁺ and salinity had significant effect on tgryptase. Ca²⁺, Mg²⁺ and salinity had significant effect on ATP enzymes(P<0.05), and Ca²⁺, Mg²⁺ and salinity had significant effect on Na⁺-K⁺-ATPase, with maximum activity at Ca²⁺ 300 mg/L, Mg²⁺ 500 mg/L and salinity 30. Ca²⁺ and Mg²⁺ had significant effect on Mg²⁺-ATPase and Ca²⁺ had significant effect on Ca²⁺-ATPase. Ca²⁺, Mg²⁺ and salinity had significant effect on immune enzymes(P<0.05), and Ca²⁺, Mg²⁺ and salinity had significant effect on ACP, with maximum activity at Ca²⁺ 100 mg/L, Mg²⁺ 150 mg/L and salinity 30. Mg²⁺ had significant effect on AKP with maximum activity at 150 mg/L. Ca²⁺ and salinity had significant effect on SOD, with maximum activity at Ca²⁺ 100 mg/L, salinity 35. The interaction of Ca²⁺, Mg²⁺, and salinity also had some impact on metabolic enzymes of L. vannamei.

Key words: Litopenaeus vannamei Ca²⁺ Mg²⁺ salinity digestive enzymes ATP enzymes immune enzymes