| 摘要: |
| 以副溶血性弧菌VP-X-3 为试验对象, 利用气相色谱技术分析了其细胞脂肪酸的组成和含量,以色谱峰的数量、色谱峰高及脂肪酸含量作为评价指标, 探讨了不同的皂化和甲酯化条件对细菌细胞脂肪酸测定结果的影响。结果表明: 皂化和甲酯化条件都对细胞脂肪酸的组成和含量产生一定的影响。较低温度皂化处理利于增加色谱峰数量和色谱峰高。甲酯化温度过高容易破坏脂肪酸, 尤其是长链脂肪酸和不饱和脂肪酸的结构。高温甲酯化条件下, 时间越长, 甲酯化效果越差。试验得到较为理想的气相色谱前处理条件: 2 mol/L NaOH-甲醇溶液2 mL, 70 ℃水浴10 min; 10%(V/V)H2SO4-甲醇溶液2 mL, 70 ℃水浴15 min。此条件下的色谱峰的数量多达29 种, 色谱峰高明显高于其他处理条件下的峰高。 |
| 关键词: 副溶血性弧菌 脂肪酸 气相色谱 甲酯化 皂化 |
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| 基金项目:国家科技支撑计划“海洋水产食品加工技术研发与产业化示范(2012BAD28B05)资助 |
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| Analysis of Cellular Fatty Acid Composition of Marine Bacteria by Gas Chromatography |
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| Abstract: |
| The composition and percentage of fatty acids in Vibrio parahaemolyticus VP-X-3 were analyzed by gas chromatography. With quantity, peak height and percentage of fatty acids showed in the chromatography as the standard, the saponification and methyl esterification conditions were also investigated to give an optimum pretreatment method. The results indicated that both saponification and methyl esterification influenced fatty acids composition and percentage. The peak quantity and height were increased by lowering saponification temperature. Too high methyl esterification temperature would easily damage fatty acids, especially the structure of long-chain fatty acids and unsaturated fatty acids. The esterification effect would be worse with longer esterification time at higher esterification temperature. The optimum gas chromatography pretreatments were as follows: saponification, 2 mL 2 mol/L NaOH-methanol, water bath at 70 for 10 min; methyl esterification, 2 ℃ mL 10% H2SO4-methanol, water bath at 70 ℃ for 15 min. On the chromatogram of GC, the quantity of chromatographic peak was as many as 29, and the peak height was distinctly higher than those with other different pretreatments. |
| Key words: Vibrio parahaemolyticus Fatty acid Gas chromatography Methyl esterification Saponification |
