| 摘要: |
| 采用响应曲面分析法研究了氯化钙水提法从海带废渣中提取岩藻聚糖硫酸酯的制备技术。在单因素试验的基础上, 根据Box-Behnken 试验设计原理, 通过建立数学模型对提取温度、提取时间、料液比等因素进行显著性和交互性分析研究, 获得了最佳提取制备参数。结果表明, 提取温度68℃, 提取时间6 h, 料液比1︰80 时, 可以获得3.95%的岩藻聚糖硫酸酯。其中岩藻糖含量为17.47%, 硫酸根含量为37.14%。 |
| 关键词: 岩藻聚糖硫酸酯 海带废渣 响应面法 |
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| 基金项目:海洋公益性行业科研专项(201105029-9) |
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| Extraction of fucoidan from Laminaria japonica residue |
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| Abstract: |
| Response surface methodology (RSM) was used to optimize the extraction of fucoidan by calcium chloride solution from Laminaria japonica residue. On the basis of single factor experiment, mathematical modeling was established to analyze the significance and interactions between extraction temperature, extraction time and material/liquid ratio. The optimum extraction conditions were extraction temperature of 68℃, extraction time of 6 h and material/liquid ratio of 1︰80 (g︰mL). Under this optimum condition the extraction rate was up to 3.95%. The contents of fucose and sulfate group were 17.47% and 37.14%, respectively. |
| Key words: fucoidan Laminaria japonica residue RSM |
