引用本文:	韦韬,顾文辉,李健,张波,潘光华,朱大玲,王广策.不同碳氮浓度对雨生红球藻生长及虾青素累积的影响[J].海洋科学,2012,36(11):55-61.

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不同碳氮浓度对雨生红球藻生长及虾青素累积的影响
韦韬¹, 顾文辉^2,3, 李健¹, 张波¹, 潘光华¹, 朱大玲¹, 王广策²
1.天津科技大学海洋科学与工程学院;2.中国科学院海洋研究所;3.中国科学院研究生院

摘要:

研究了不同二氧化碳浓度和硝酸钾浓度对雨生红球藻生长及虾青素累积的影响。结果表明, 较高浓度的CO₂(600×10^-6)能够显著促进雨生红球藻的生长、光合作用的进行和虾青素的累积。红球藻单个细胞内的虾青素含量随着培养液中硝酸钾浓度的降低而增加, 绿色游动藻种和绿色不动藻种培养12 d 后获得的最大虾青素值分别为10.93 pg/个和12.64 pg/个。连续通气是促进雨生红球藻生长及虾青素累积的一种有效碳源提供方式。

关键词: 雨生红球藻虾青素二氧化碳硝酸钾

DOI：

分类号:

基金项目:国家自然科学基金项目(30970302, 41176137, 41176134,41106131); 973 前期启动项目(2011CB411908); 青岛市科技计划基础研究项目(11-2-4-8-(5)-jch); 天津市科技支撑计划项目(10ZCKFSH00700)

Effects of carbon and nitrogen on the growth and astaxanthin accumulation of Haematococcus pluvialis

Abstract:

Haematococcus pluvialis is a green microalga which accumulates the highest astaxanthin content among all the potential organism sources. In this work, the effects of different concentrations of CO₂ and KNO₃ on the growth and astaxanthin accumulation of H. pluvialis were studied. The results showed that CO₂(600×10^-6 could significantly promote the growth, photosynthesis and astaxanthin accumulation of H. pluvialis. The astaxanthin content within a single cell was increased by the decrease of KNO₃ concentration of the medium. The maximum astaxanthin content of green vegetative cell and green rest cell were 10.93 and 12.64 pg/cell respectively after cultured for 12 days. Continuous ventilation is an effective way to provide carbon source for the growth and astaxanthin accumulation in H. pluvialis.

Key words: Haematococcus pluvialis astaxanthin CO₂ KNO₃