| 摘要: |
| 利用ISSR 分子标记技术对采自浙江省南麂岛和渔山列岛的4 株不同形状野生坛紫菜(Porphyra haitanensis)进行了遗传多样性分析。从33 条引物中筛选出11 条可扩增出清晰条带的引物, 在4 株坛紫菜中共扩增出62 条DNA 带, 其中多态性条带比例达 88.71%。遗传距离分析表明4 株坛紫菜之间的遗传距离范围是0.3226～0.6129, 聚类分析结果表明渔山列岛坛紫菜遗传距离最近, 而南麂岛的两株坛紫菜则距离相对较远。该研究说明浙江附近岛屿的野生坛紫菜遗传多样性丰富。 |
| 关键词: 坛紫菜(Porphyra haitanensis) ISSR 遗传多样性 |
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| 基金项目:国家科技支撑计划项目(2011BAD13B08); 国家公益性行业(海洋)科研专项经费项目(201105009); 国家公益性行业(农业)科研专项经费项目(201003068) |
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| Genetic diversity analysis of four Zhejiang coastal Porphyra haitanensis strains with ISSR marker |
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| Abstract: |
| The genetic diversity of four morphologically different Porphyra haitanensis strains collected from Nanji Island and Yushan Island of Zhejiang Province was analyzed with ISSR markers. In total, 11 primers were selected out from 33 primers for diversity analysis. These primers amplified 62 bands, and 88.71% of the bands were polymorphic. The genetic distance of the four strains of P. haitanensis ranged from 0.3226 to 0.6129. Two strains from Nanji Island were clustered together first, with other two strains Yushan Island joined then, indicating that the wild P. haitanensis along Zhejiang Provincial coast is rich in diversity. |
| Key words: Porphyra haitanensis ISSR genetic diversity |
