| 摘要: |
| 利用超声波破碎技术在1 mol/L HCl-CH3OH 溶液中提取小球藻脂肪酸并酯化, 并用气相色谱和气质联用仪对其进行定量和定性分析, 鉴定出C14:0, C16:2, C16:1, C16:0, C18:2, C18:1, C18:3, C18:0,C20:0 共9 种脂肪酸, 其中不饱和脂肪酸占总脂肪酸的65%。6 份平行样的相对标准偏差为6.04%～9.84%, 样品加标回收率范围为77.0%～122.6%, 各种脂肪酸甲酯的仪器检测限＜0.3 ng。初步探讨了脂肪酸甲酯在弱极性色谱柱上的保留时间规律。 |
| 关键词: 小球藻 脂肪酸 气相色谱 气质联用仪 保留时间 |
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| 基金项目:国家教育部高等学校骨干教师计划基金资助项目; 厦门大学预研基金资助项目(B 类2003xdyy40); 国家海洋局公益性项目(201005012) |
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| Determination of fatty acids in algae Chlorella by gas chromatography |
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| Abstract: |
| A shortcut method to analyze fatty acids in marine algae quickly and easily was reported in the paper. The ultrasonic extraction was used in the pretreatment of algae Chlorella, then the fatty acids were converted to fatty acid methyl esters by 1 mol/L HCl-CH3OH. The composition of fatty acid methyl esters was analyzed with gas chromatography and gas chromatography/mass spectrometry. Nine fatty acids including C14:0, C16:2, C16:1, C16:0, C18:2, C18:1, C18:3, C18:0, C20:0 were found and the unsaturated fatty acids was 65% of total. The relative standard deviations of six parallel samples were 6.04%～9.84%. The recoveries for spiked standards in samples were 77.0%～122.6%. The limits of detection for instrument were below 0.3 ng. The rule of retention time for fatty acid methyl ester was investigated. This method is very simple, quick and accurate and could be used in practical application. |
| Key words: Algae Chlorella Fatty acids Gas chromatography Gas chromatography/mass spectrometry Retention time |
