| 摘要: |
| CTAB-酚/氯仿法提取新鲜凡纳滨对虾(Litopenaeus vannamei)肠道微生物宏基因组DNA。结果表明: CTAB-酚/氯仿法提取总DNA 的浓度达到92.5 ng/μL, 半定量PCR 法检测微生物基因组相对含量为69.9%。凡纳滨对虾肠道微生物宏基因组用于Solexa 测序, 生物信息学分析结果显示宏基因数据中64.1%的数据属于未知序列, 35.5%属于真核生物, 而已知的微生物和病毒序列所占比例仅有0.4%。 |
| 关键词: 肠道微生物宏基因组 CTAB Solexa 测序 |
| DOI： |
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| 基金项目:国家高技术研究发展计划(2007AA09Z444) |
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| Solexa sequencing and analysis of the intestinal microorganisms metagenome in Litopenaeus vannamei |
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| Abstract: |
| The intestinal microorganisms metagenome of Litopenaeus vannamei was extracted by CTAB-phenol/ chloroform DNA extraction protocol. The relative concentration of bacterial genome in metagenome was analyzed by semi-quantitative PCR. The results showed that the total concentration of metagenome was 92.5 mg/L and relative microbial metagenome ratio was 69.9%. Solexa sequencing showed that up to 64.1% of contigs in metagenome had no assignment or no hits in database of NCBI, 35.5% of which were assigned to eukaryon, and only 0.4% sequences belonged to bacteria and virus. |
| Key words: intestinal microfloar metagenome CTAB solexa sequencing |
