| 摘要: |
| 采用亚甲基蓝显色分光光度法测定海参不同组织酸性黏多糖含量。在pH 为5～6 的酸性条件下,海参多糖以阴离子状态存在, 能够与亚甲基蓝发生异染现象, 生成的异染配合物在564 nm 处有最大吸收波长; 在4～20 mg/L 范围内呈现良好的线性关系, 方法回收率为78.33%～100.09%, 精密度＜10%,该方法最低检出浓度为0.79 mg/L。利用该方法测定了海参不同组织酸性黏多糖含量, 结果表明海参体壁中酸性黏多糖含量最高, 海参卵次之, 海参肠最少。 |
| 关键词: 海参酸性黏多糖 亚甲基蓝 分光光度法 |
| DOI： |
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| 基金项目:中央级科研院所基础科研经费项目(2008-GY-08, 2009-CB-02) |
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| Spectrophotometric determination of mucopolysaeccharide from different parts of sea cucumber with methylene blue |
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| Abstract: |
| Mucopolysaeccharide from different parts of sea cucumber was measured by spectrophotometrically at 564 nm with methylene blue. In the pH range of 5～6, the anionic mucopolysaeccharide reacted with methylene blue. The absorbance was in good linearity in the concentration range of 4～20 mg/L. The recoveries of this method were in the range of 78.33%～100.09%, the RSD＜10%, and the limits of detection (LODs) 0.79mg/L. The results showed that the content of acid mucopolysaeccharide in the body was the highest, followed in turn by those of the ovum and the intestine. |
| Key words: seacucumber acid mucopolysaccharide methylene blue spectrophotometry |
