| 摘要: |
| 运用随机扩增片段长度多态性DNA 标记(RAPD)技术对中国海南省英州、安游和亚龙湾海域的3个羊鲍(Haliotis ovina)野生群体进行遗传多样性与分化的研究。利用14 个10bp 随机引物, 在90 个个体中共检测到72 个位点, 其中英州, 安游和亚龙湾群体的多态位点数分别为65, 61 和62 个, 多态位点百分数(P)分别为90.28％、84.72％和86.11％; Shannon’s 信息指数(I)分别为0.443、0.456 和0.400;Nei’s基因多样性指数(H)分别为0.292、0.305 和0.262。3 个羊鲍群体间的遗传距离(D)介于0.0504～0.0731之间; 群体间的遗传分化系数(GST)为0.0976。结果表明, 羊鲍3 个野生群体的遗传多样性水平较高, 群体间的遗传分化较小。 |
| 关键词: 羊鲍(Haliotis ovina) 遗传多样性 遗传分化 RAPD |
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| 基金项目:国家自然科学基金项目(30271013); 国家留学回国人员科研启动基金[教外司留(2006)331 号]; 福建省高等教育新世纪优秀人才支持计划资助项目[闽教科〔2006〕35]; 集美大学创新团队基金资助项目(2007A001) |
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| Genetic diversity and differentiation of three wild populations of Haliotis ovina |
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| Abstract: |
| Random amplified polymorphic DNA (RAPD) technique was used to analyze three wild Haliotis ovina from Yingzhou, Yalongwan, and Anyou of Hainan Province. Seventy-two loci were found by 14 random primers, and Yingzhou, Yalongwan, Anyou populations having 65, 61 and 62, respectively. The percentages of polymorphic loci (P) of the three populations were 90.28%, 84.72%, and 86.11%, respectively; the Shannon’s information index (I) was 0.443 in Yingzhou population, 0.456 in Yalongwan population, and 0.400 in Anyou population; the Nei’s gene diversity (H) were 0.292, 0.305, and 0.262, respectively. Genetic distances(D)among the three populations were about 0.0504～0.0731. The genetic differentiation index GST among the three populations was 0.0976. Our study shows that the genetic diversity of H. ovina is at a high level, and the genetic differentiation is low. |
| Key words: Haliotis ovina genetic diversity genetic differentiation RAPD |
