引用本文:	刘国辉,吴后波,李翔,颜文.海南岛东部501 站位沉积物古细菌多样性研究[J].海洋科学,2011,35(2):86-91.

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海南岛东部501 站位沉积物古细菌多样性研究
刘国辉^1,2, 吴后波¹, 李翔¹, 颜文^3,4
1.中国科学院南海海洋研究所LMB实验室;2.中国科学院研究生院;3.中国科学院广州天然气水合物研究中心;4.中国科学院边缘海重点实验室

摘要:

从海南岛东部501 站位沉积物中扩增古菌16S rRNA 基因构建文库, 并对得到的OTU(Operation taxonomic units)进行系统发育学分析。古菌文库由泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota)构成, 其中泉古生菌占总克隆文库的96.8%, 由Ⅰ型海洋泉古生菌(Mraine crenarchaeotic otic groupⅠ,MGⅠ, 74.6%)、GROUP A(14.9%)和GROUP B(7.2%)三簇组成。广古生菌GROUP C 簇占总克隆文库的3.2%, 序列比对结果显示它们与甲烷微菌纲(Methanomicrobial)中甲烷厌氧氧化菌(ANME)和甲烷八叠球菌目(Methanosarcinales)有较近的系统发育学关系。研究表明海南岛东南部近岸沉积物古菌多样性比较丰富, 泉古生菌是该站位古菌的优势菌。

关键词: 海洋沉积物 16S rDNA 古菌

DOI：

分类号:

基金项目:中国科学院知识创新工程重要方向项目(KZCX2-YW-211-03); 中国科学院南海海洋研究所创新领域前沿项目(lyqy200312)

Phylogenetic analysis of archaeal community from the sediment at location 501 at the east of Hainan island

Abstract:

Diversity of archaea was studied in marine sediment by PCR amplification and sequence analysis of 16S rRNA. Samples were from a site at the east of Hainan island. The phylogenetic analysis revealed that archaeal population was composed of Crenarchaeota and Euryarchaeota. Mraine crenarchaeotic group (74.6%), GRⅠ OUP A(14.9%) and GROUP B(7.2%) fall into Crenarchaeota. The GROUP C sequences were closely related to ANME and Methanosarcinales. The result indicates that archaea are diversified in surface environment of subseafloor sediment and Crenarchaeota is the predominant aechaea.

Key words: marine subseafloor sediment archaea