| 摘要: |
| 以7 种水产病原菌为指示菌, 采用菌饼法对分离自南麂岛海域的15 个沉积物样品中的78 株海洋真菌进行抑菌活性初筛, 获得了对至少一种病原指示菌有抑菌活性的菌株19 株。采用打孔扩散法对这19 株真菌的发酵液进行抑菌活性复筛, 共有9 个菌株的次级代谢产物对一种或者几种病原指示菌有抑制作用, 其中菌株NJ0104 的次级代谢产物对除灿烂弧菌(Vibrio splendidus)之外的6 种指示菌均有抑制作用, 尤以对副溶血弧菌(Vibrio parahaemolyticus)的抑制活性为最强。对菌株NJ0104 次级代谢产物中抑菌活性物质的初步研究表明, 抑菌活性物质在80 ℃以下、紫外照射40 min 时稳定性良好, pH 稳定范围在4.0～7.0 之间, 能较好地溶解于正丁醇中。结果表明: 南麂岛海域的沉积物中分布着丰富的真菌资源, 分离得到的海洋真菌NJ0104 具有较高的研究价值, 值得深入研究其活性成分及抑菌机理。 |
| 关键词: 南麂岛 海洋沉积物 海洋真菌 抑菌活性 理化性质 |
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| 基金项目:温州市科技计划资助项目(Y20080092); 浙江省科技厅面上项目(2009C33004) |
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| Isolation of antibacterial fungus from sediment of Nanji Island and properties of its metabolites |
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| Abstract: |
| From fifteen sediment samples collected from the Nanjidao sea area, 78 marine fungal strains were isolated and evaluated against seven types of pathogenic vibrio from mariculture organisms. The antibacterial screening showed that nineteen strains could inhibit at least one pathogenic vibrio, and nine of these produced antibacterial metabolites, which had activity against one or several types of pathogenic vibrio. The strain NJ0104 with wide antimicrobial spectrum had the strongest activity against Vibrio parahaemolyticus. A preliminary study of antibacterial metabolites produced by NJ0104 demonstrated that they had preferable thermal stability at 80℃, ultraviolet stability within forty minutes and pH stability at 4.0～7.0, and antibacterial substances could be well dissolved in butanol. The active ingredients and antibacterial mechanism of NJ0104 were worth further intensive study. |
| Key words: Nanji Island marine sediment marine fungi antibacterial activity physical and Chemical properties |
