| 引用本文: | 高凤祥,郭 文,潘 雷,胡发文,菅玉霞,张少春,王 雪.几种益生菌对大菱鲆幼鱼生长及消化酶活性的影响[J].海洋科学,2011,35(1):10-16. |
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| 摘要: |
| 作者以初体质量为(4.80±0.11)g 的大菱鲆(Scophthalmus maximus)为研究对象, 在循环水水族箱中进行60 d 饲喂实验, 研究饵料中添加单一益生菌和复合益生菌对大菱鲆生长及消化道酶活性的影响。以基础饲料为对照组, 在基础饲料中分别添加枯草芽孢杆菌(Bacillus subtilis)、地衣芽孢杆菌(Bacillus licheniformis)、复合芽孢杆菌组(1 : 1)、嗜酸乳酸杆菌(Lactobacillus acidophilus)、双歧杆菌(Bifidobacterium inopinatum)、复合乳酸菌组(1 : 1)、复合益生菌(1 : 1 : 1 : 1), 每克饲料约含菌3.0×109个/g 干质量, 共配制7 种实验饲料。实验结果显示, 复合益生菌饲喂组大菱鲆的存活率和特定生长率显著高于对照组(P＜0.05), 而单一益生菌饲喂组与对照组差异不显著(P＞0.05)。饲料中添加益生菌能使大菱鲆消化道蛋白酶、脂肪酶和淀粉酶活力升高, 其中添加复合益生菌的实验组消化道酶活性显著高于对照组(P＜0.05)。上述实验结果表明, 与饲喂单一益生菌相比, 添加复合益生菌对提高大菱鲆存活率, 促进大菱鲆的生长具有更显著的促进作用。 |
| 关键词: 大菱鲆(Scophthalmus maximus) 益生菌 生长 消化酶活性 |
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| 基金项目:国家海洋局公益性专项项目(200705023) |
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| Effects of dietary probiotics on growth and digestive enzyme activities of juvenile Scophthalmus maximus |
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| Abstract: |
| Experiments were conducted to determine the effects of different dietary administration of Probiotics on growth (SR: survival rate; SGR: special growth rate) and digestive enzyme activitis (including proteases、lipase and amylase) of Scophthalmus maximus. The initial body weight was(4.80±0.11), and the period of the experiment was 60 d. Basic diet was made of fish meal and defatted soybean meal as main protein sources. These juvenile turbots were fed with eight different diets: basic diet as control, and basic diet supplemented with 3.0×109CFU/g single probiotics (Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Bifidobacterium inopinatum) or 3.0 ×109CFU/g compound probiotics (B.licheniformis + B.subilis 1 : 1; B. inopinatum + L. acidophilus 1 : 1; B.licheniformis + B.subilis + B. inopinatum + L. acidophilus 1 : 1 : 1 : 1). Each diet was randomly fed to triplicate groups of 300 turbots reared in circulating water aquarium. The water temperature was 17℃±1℃ and the salinity was 32±0.2 during the experimental period. The results showed that turbot fed compound probiotics showed relatively higher growth rate compared with those fed control diet (P＜0.05). However, no significant differences in growth were observed between single probiotic groups and the control group. Diet with supplementation of probiotics could improve the protease、lipase and amylase activities in digest tract of turbot, that is, the activities of protease was significantly higher in turbots fed the compound probitics (P＜0.05) than those in turbots fed control diet. The present results indicate that the diet with supplementation of probiotics can improve growth and digestive enzyme activities, and that compound probiotics groups show better effects than single probiotics groups. |
| Key words: Scophthalmus maximus probiotics growth digestive enzyme activity |
