| 摘要: |
| 采用16S rDNA 文库结合PCR-RFLP 分析的手段, 对胶州湾4 个站位沉积物中的细菌多样性和群落特征研究分析。结果表明, 沉积物中细菌种类丰富, 最多包含分布于14 个已知门类的细菌, 和一些未被认知的序列; 各站位沉积物中优势菌群均为变形菌门和酸杆菌门, 其中γ-和δ-变形菌纲为变形菌门中的绝对优势类群, 在4 个文库序列中平均占42%和16.75%; 此外, 拟杆菌门、浮霉菌门和放线菌门的种类也较为大量存在。各细菌种群有较明显空间分布差异, 可能与不同区域胶州湾环境条件相关。 |
| 关键词: 沉积物 细菌群落 16S r DNA 多样性 胶州湾 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(30770234, 30910103914); 山东省科技发展计划项目(2006GG2205023) |
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| Diversity of bacterial community in sediments of Jiaozhou Bay |
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LIU Xin,XIAO Tian,ZHANG Wen-yan,DONG Yi,YUE Hai-dong1
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| Abstract: |
| The 16S rDNA clone library technique and PCR-RFLP analysis were applied to give a comprehensive view of the diversities and compositions of sediment bacterial communities at four sampling stations of Jiaozhou Bay. The 16S rDNA clone library analysis indicated that the sediments contained high diversities of bacteria and distributed in 15 known phyla in maximum, and some sequences were affiliated to uncultured bacteria. Proteobacteria and Acidobacteria were the dominant groups in the sediments. γ-Proteobacteria and δ-Proteobacteria, averagely accounting for 42% and 16.75% of the four libraries, respectively, were the most dominant classes in Proteobacteria. Members of Bacteroidetes, Planctomycetes and Actinobacteria were also comparatively dominant groups found in Jiaozhou Bay sediments. Bacterial community diversities differed in space, probably resulting from the special habitats of different microbial groups and also the different geographical locations in Jiaozhou Bay. |
| Key words: sediment microbial community 16Sr DNA diversity Jiaozhou Bay |
