引用本文:	王清,杨红生,王晓宇.镉和苯并芘胁迫对文蛤血细胞功能的影响[J].海洋科学,2010,34(9):82-86.

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镉和苯并芘胁迫对文蛤血细胞功能的影响
王清^1,2, 杨红生¹, 王晓宇³
1.中国科学院海洋研究所;2.中国科学院研究生院;3.河北工业大学海水资源高效利用化工技术教育部工程研究中心

摘要:

应用流式细胞仪技术, 通过检测血细胞的吞噬活力、活性氧产物和死亡率, 研究了Cd²⁺和苯并芘(Bap)胁迫7 d 对文蛤血细胞功能的影响。结果表明, 10 μg/L 和100 μg/L Cd²⁺胁迫会显著降低血细胞的吞噬活力, 增加血细胞的死亡率, 100 μg/L Cd²⁺会显著增加血细胞活性氧产物。5 μg/L 和50 μg/L Bap胁迫会显著降低文蛤血细胞的吞噬活力, 增加血细胞的死亡率, 50 μg/L Bap 会显著增加血细胞活性氧产物。可见, Cd²⁺和苯并芘胁迫会显著降低文蛤血细胞的功能, 导致文蛤免疫力下降, 增大文蛤被病害感染的机率。

关键词: 文蛤(Meretrix meretrix) 血细胞镉苯并芘

DOI：

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基金项目:国家基金委创新研究群体科学基金项目(40821004); 国家973 计划资助项目(2007CB407305); 国家农业部公益性计划资助项目(nyhyzx07-047); 国家海洋公益性行业科研专项资助项目(200805069)

The effects of cadmium and benzo[α]pyrene exposure on the immune function of Meretrix meretrix hemocytes

WANG Qing,YANG Hong-sheng,WANG Xiao-yu

Abstract:

The effects of cadmium and benzo[α]pyrene (Bap) exposure on the immune function of Meretrix meretrix hemocytes were examined by flow cytometery. The results showed that 10 and 100 μg/L Cd²⁺ significantly suppressed phagocytosis of hemocytes and increased the mortality of hemocytes, while 100 μg/L Cd²⁺ stimulated reactive oxygen species (ROS) in hemocytes. Bap (5 and 50 μg/L) inhibited phagocytosis and increased the mortality of hemocytes, while 50 μg/L Bap could enhance ROS. The results suggested that cadmium and benzo[α]pyrene exposure significantly inhibited the immune function of hemocytes, which may lead to low immunity and increased infection of M. meretrix.

Key words: Meretrix meretrix hemocyte cadmium benzo[α]pyrene