引用本文:	徐年军,何艳丽,武敏,严小军.大米草多糖的提取及其单糖组成的GC-MS 分析[J].海洋科学,2010,34(9):15-18.

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大米草多糖的提取及其单糖组成的GC-MS 分析
徐年军^1,2, 何艳丽^1,2, 武敏^1,2, 严小军^1,2
1.宁波大学生命科学与生物工程学院;2.教育部应用海洋生物技术重点实验室

摘要:

分析了海洋滩涂生物大米草(Spartina anglica)中的多糖成分和单糖组成, 为这种生物资源的开发提供科学依据。利用水提醇沉、Sevag 法脱蛋白得到纯大米草多糖, 经三氟乙酸降解、糖腈乙酸酯衍生,最后用气相色谱-质谱联用法(GC-MS)分析大米草多糖中的单糖组成。结果表明大米草单糖组成为鼠李糖、核糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖, 其质量分数分别是: 4.13%, 3.02%, 11.94%, 8.89%,6.82%, 27.18%, 38.00%。大米草多糖是一种可利用的食品资源, 其主要成分半乳糖、葡萄糖都是营养价值很高的单糖, 大米草多糖可深入研究开发其生物活性用作保健食品。

关键词: 大米草(Spartina anglica) 多糖单糖组成 GC-MS 分析

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基金项目:浙江省卫生厅项目(2005B149); 浙江省自然科学基金项目(Y507263); 浙江省钱江人才计划项目(2007R10038); 宁波市自然科学基金项目(2007A610071)

Extraction of polysaccharides from Spartina anglica and analysis of the monosaccharide composition by gas chromatography-mass spectrometry

XU Nian-jun,HE Yan-li,WU Min,YAN Xiao-jun

Abstract:

To study the polysaccharides and analysis their monosaccharide compositions of the Spartina anglica, purified polysaccharides were obtained by hot water extraction, ethanol precipitation, and de-protein by Sevag method. The polysaccharides were hydrolyzed to monosaccharides by 2 mol/mL trifluoroacetic acid in 120℃ oil-bath for 1h. The derived monosaccharides were analyzed by gas chromatography-mass spectrometry (GC-MS). The S. anglica polysaccharides consisted of rhamnose, ribose, arabinose, xylopyranose, mannose, glucose, lactose, with their relative percentages being 4.13%, 3.02%, 11.94%, 8.89%, 6.82%, 27.18%, and 38.00%, respectively. It is predicted that the polysaccharides of the S. anglca are a potential food resource, with the main monosaccharides, i.e. lactose and glucose, being of very high nutritional value. Further study for their applications in functional foods is warranted.

Key words: Spartina anglica polysaccharides monosaccharide composition GC-MS