| 摘要: |
| 本试验测定了条斑星鲽(Verasper moseri)幼鱼变态过程中DNA、RNA、蛋白及其比值的变化, 作为评价幼鱼变态阶段生长潜能的指标。结果表明, 试验期间培育水温为16.5～18.0°C, DNA、RNA及蛋白的变化都带有发育阶段特异性。DNA 含量在34～42日龄增加缓慢, 然后快速增加直至44日龄, 在45日龄和46日龄分别保持下降和上升趋势。蛋白含量和RNA含量的变化趋势相似, 在试验期间保持快速增长的趋势。RNA/DNA比值从试验开始至35日龄呈上升状态, 36日龄时下降, 38日龄时达到最高值5.01, 然后显著下降(P<0.05), 在试验结束时达到最低值1.76。蛋白/DNA 在40日龄时达到最高值58.64, 在46日龄时达到最低值21.28。RNA、DNA及蛋白含量跟全长和体质量有明显的线性关系(P<0.05)。RNA/DNA与全长和体质量的关系比蛋白/DNA与全长和体质量的关系密切。蛋白/DNA比值的变化趋势跟RNA/DNA比值类似, 但前者的变化滞后于后者, 表明RNA/DNA比值是评价条斑星鲽生理状况的更有效的生理指标。
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| 关键词: 条斑星鲽(Verasper moseri) 幼鱼 DNA RNA 蛋白 |
| DOI： |
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| 基金项目:现代农业产业技术体系建设专项资金资助项目(nycytx-50);国家863“十一五”计划资助项目(2006AA10A404) |
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| Changes in nucleic acids and protein indices during metamorphosis of barfin flounder Verasper moseri |
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TONG Xue-hong,XU Shi-hong,LIU Qing-hua,LI Jun,XIAO Zhi-zhong,MA Dao-yuan
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| Abstract: |
| Changes in DNA, RNA, protein and their ratios of barfin flounder Verasper moseri during metamorphosis were determined, and were used to evaluate the growth potential of barfin flounder at this stage. Water temperature fluctuated between 16.5°C and 18.0°C. Patterns of DNA, RNA and protein parameters all showed developmental stages-specific traits. DNA content remained slight increase until 42 days after hatching (DAH) and showed abrupt rise towards 44 DAH followed by a drop and a rise around 45 and 46 DAH, respectively. The changes in protein content coincided well with those of RNA content; and they both kept obvious increases throughout the experimental period. RNA/DNA ratio increased up to 35 DAH, but decreased at 36 DAH, and reached the climax of 5.01 at 38 DAH, which was followed by a significant decline with the minimum value of 1.76 at the end of the experiment. Protein/DNA reached the maximum value of 58.64 at 40 DAH and the minimum value of 21.28 at 46 DAH. DNA, RNA and protein contents all showed significant correlations with total length and body weight of barfin flounder (P<0.05). RNA/DNA had stronger correlations with total length and body weight of barfin flounder than protein/DNA. Protein/DNA showed a similar pattern to RNA/DNA, but the changes in protein/DNA lagged behind those in RNA/DNA, suggesting that RNA/DNA ratio is a more sensitive index than protein/DNA ratio in evaluating physiological conditions of barfin flounder.
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| Key words: barfin flounder (Verasper moseri) juvenile DNA RNA protein |
