引用本文:	刘艳如,庄惠如,田宝玉,江贤章,张国海.一株海洋金藻的18S rRNA 基因序列分析及分类[J].海洋科学,2010,34(4):53-57.

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一株海洋金藻的18S rRNA 基因序列分析及分类
刘艳如¹, 庄惠如¹, 田宝玉¹, 江贤章¹, 张国海¹
福建师范大学生命科学学院

摘要:

Isochrysis sp. strain HG 是一株分离自福建长乐自然海区的金藻, 是一种良好的西施舌育苗饵料藻。显微形态观察表明, Isochrysis sp. strain HG 的形态特性与等鞭金藻属的球等鞭金藻(I. galbana)和湛江等鞭金藻(I. zhanjiangensis)比较接近。进一步克隆Isochrysis sp. strain HG 的核糖体小亚基(small subunit, SSU)18S rRNA 基因, 获得了长度为1 780 bp 的基因序列。同源性分析表明, 该序列与在NCBI数据库中登录的等鞭金藻属的18S rRNA 基因序列同源性最高, 说明结果与形态鉴定的结果相一致。基于18S rRNA 基因序列的系统发育分析表明, Isochrysis sp. strain HG 与等鞭金藻属的I. zhangjiangensis、Isochrysis sp. strain CCAP927/14、I. galbana 和Isochrysis sp. strain Santou 聚在一个分支类群上, 其中I. zhangjiangensis 与Isochrysis sp. strain CCAP927/14 聚为一个独立的子类群, 和I. galbana、Isochrysis sp. strain Santou 以及目标菌株Isochrysis sp. strain HG 形成4 个并列独立的分支。根据形态及分子系统分析结果, Isochrysis sp. strain HG 可能是不同于I. zhangjiangensis、I. galbana 的等鞭金藻种类。

关键词: Isochrysis sp. strain HG 鉴定 18S rDNA 系统发育分析

DOI：

分类号:

基金项目:福建省科技计划重点项目(2006N0038)

Identification and sequence analysis of the 18S rRNA gene of a marine microalgae

LIU Yan-ru,ZHUANG Hui-ru,TIAN Bao-yu,JIANG Xian-zhang,ZHANG Guo-hai

Abstract:

Isochrysis sp. strain HG, a potentially valuable bait-microalgae for Coelomactra antiquata, was isolated from the natural water of Changle, Fujian, China. It showed extremely similar morphological characteristics with the strains of Isochrysis genera I. galbana and I. zhanjiangensis under the microscopes. The 18S rRNA gene of Isochrysis sp. strain HG was cloned to further identify the strain at the molecular level. The homologous analysis shown that the 1780 bp derived from the 18S rDNA sequence was highly homologous to the relevant sequences of I. galbana and I. zhanjiangensis in GenBank. Furthermore, the phylogenetic tree based on the 18S rDNA Sequences was constructed, showing that the Isochrysis sp. strain HG, I. Zhangjiangensis, Isochrysis sp. strain CCAP927/14, I. galbana, and Isochrysis sp. strain Santou, were all clustered with together. Among them, I. zhangjiangensis subgroups with Isochrysis sp. strain CCAP927/14, and I. galbana, and Isochrysis sp. strain Santou;and Isochrysis sp. strain HG was further formed one of four subclades. Based on the morphological and molecular evidence, Isochrysis sp. strain HG was proposed to be a candidate species of Isochrysis genera, which is different from the other three Isochrysis species that have been previously described.

Key words: Isochrysis sp. strain HG identification 18S rDNA phylogenetic analysis