引用本文:	薛丁萍,魏玉西,刘淇,刘蕾.浒苔多糖对羟自由基的清除作用研究[J].海洋科学,2010,34(1):44-47.

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浒苔多糖对羟自由基的清除作用研究
薛丁萍¹, 魏玉西¹, 刘淇², 刘蕾¹
1.青岛大学生物系;2.中国水产科学研究院黄海水产研究所

摘要:

研究确定了Fenten(水杨酸法)反应所需水杨酸、H₂O₂和Fe²⁺的浓度，并用水杨酸法对浒苔(Enteromorpha prolifera)水提多糖及其酸提多糖对羟自由基的清除能力进行了测定，同时，与合成抗氧化剂BHT和TBHQ进行对比。结果表明，浒苔水提多糖及酸提多糖对羟自由基均有一定的清除效果，且清除率与浓度存在剂量依赖关系，酸提多糖对羟自由基的清除能力大于水提多糖。在较高质量浓度(＞1.2 g/L)时酸提多糖对羟自由基的清除能力是水提多糖的2倍。

关键词: 浒苔(Enteromorpha prolifera) 多糖羟自由基清除率

DOI：

分类号:

基金项目:国家科技支撑计划项目(2008BAC49B04)；青岛市科技计划项目(08-1-7-6-hy)

Study on the scavenging activity of polysaccharides from Ente romorpha prolifera to hydroxyl radical

XUE Ding-ping,WEI Yu-xi,LIU Qi,LIU Lei

Abstract:

The concentrations of salicylic acid, H₂O₂ and Fe²⁺ needed in Fenten reaction model were exa-mined firstly in this paper. Polysaccharides extracted by water (PW) and acid (PA) from Enteromorpha were prepared, and then their anti-oxidative activities were evaluated by radicals scavenging ratio to ·OH and compared with synthesized antioxidants BHT and TBHQ. The results showed that PW and PA all had certain activities to scavenge ·OH and in a dose dependent manner, and PA presented higher scavenging ratio to ·OH than PW. At the sample concentration of more than 1.2 g/L for PA, the scavenging ratio to ·OH doubled compared with PW. Therefore, it was suggested that polysaccharide by acid extraction from Enteromorpha should be a strong ·OH scavenger and thus has a bright future in food industry.

Key words: Enteromorpha polysaccharide ·OH scavenging ratio