| 摘要: |
| 采用同源克隆的方法、利用RACE技术从黄鳍金枪鱼(Thunnus albacares)肝脏中克隆了其MIF(TaMIF)的cDNA序列。TaMIF的cDNA全长706 bp含一个345 bp的开放阅读框,编码一个长115氨基酸的蛋白质。信号肽预测分析表明TaMIF没有信号肽。序列分析与进化分析表明，黄鳍金枪鱼与近海养殖鱼类MIF的氨基酸序列高度相似、进化地位相近，预示着深海鱼类TaMIF与近海养殖鱼类具有相似的生物学功能。研究结果是对深海鱼类MIF基因信息资料的重要补充。
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| 关键词: 黄鳍金枪鱼（Thunnus albacares） 巨噬细胞移动抑制因子（MIF） 分子克隆 序列分析 |
| DOI： |
| 分类号: |
| 基金项目:福建省科技重大专项（2004NZ03) |
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| Molecular cloning and sequence analysis of macrophage migration inhibitory factor(MIF) gene from Thunnus albacares |
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MAO Yong,WANG Jun,ZHANG Zhi-wen,WANG Ding,SU Yong-quan
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| Abstract: |
| The cDNA sequence of an MIF homologue (TaMIF) was cloned from the liver of Thunnus albacares by a homologous cloning strategy and RACE approach. The full length cDNA of TaMIF was 706 bases long and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. Predication of the signal peptide indicated that TaMIF lacks a signal peptide. Sequence analysis revealed a high structural similarity and phylogenetic analysis indicated a close relationship between TaMIF and other fish MIF, implying that TaMIF maybe have a simliar function to offshore cultured fish MIFs. The result is the complement of the gene information to open sea fish MIF gene.
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| Key words: Thunnus albacares macrophage migration inhibitory factor(MIF) molecular cloning equence analysis |
