| 引用本文: | 杨奔,尤锋,李军,徐冬冬,吴志昊,于道德,倪静,徐世宏,徐永立,张培军.RAPD对圆斑星鲽和条斑星鲽养殖群体的遗传多样性研究[J].海洋科学,2009,33(8):26-29. |
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| 摘要: |
| 应用RAPD分子标记技术对圆斑星鲽(Verasper variegatus)和条斑星鲽(Verasper moseri)的养殖群体进行群体遗传多样性分析。每个群体各取鱼30尾，并从78条随机引物中筛选出20条用于群体遗传学研究。两个群体共扩增出218条DNA片段，大多数片段大小为250～1500 bp，其中圆斑星鲽185条，条斑星鲽183条。多态片段比例分别为56.76%和64.48%，Nei的多样性指数和Shannon的信息指数分别为0.232 2和0.278 1、0.339 1和0.398 9。这两种星鲽养殖群体间的遗传相似性系数和遗传距离分别为0.672 0和0.397 5。
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| 关键词: 圆斑星鲽（Verasper variegatus) 条斑星鲽（Verasper moseri) 养殖群体 RAPD 遗传多样性 |
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| 基金项目:国家863计划资助项目（2006AA10A404）；国家科技部水产种质资源整理、整合与共享种质平台项目（2006DKA30470－017） |
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| Study on genetic diversities in Verasper variegatus and Verasper moseri cultured stocks using RAPD analysis |
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YANG Ben,YOU Feng,LI Jun,XU Dong-dong,WU Zhi-hao,YU Dao-de,NI Jing,XU Shi-hong,XU Yong-li,ZHANG Pei-jun
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| Abstract: |
| The RAPD molecular technology was used to analyze the genetic diversities of cultured stocks of spotted halibut, Verasper variegatus and barfin flounder, Verasper moseri. Out of 78, 20 arbitrary oligonucleotide primers were screened and for PCR amplification for stock genetics analysis. Total 218 bands were amplified, 185 in spotted halibut and 183 in barfin flounder, most ranging from 250 to 1500 bp. The percentage values of polymorphic bands of these two stocks were 56.76% and 64.48%, and their Nei’s genetic diversity values were 0. 232 2 and 0. 278 1, respectively. Their Shannon indexes were 0.339 1 and 0.398 9, respectively. Nei’s genetic identity and genetic distance between these two Verasper cultured stocks were 0.672 0 and 0.397 5, respectively.
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| Key words: Verasper variegatus Verasper moseri cultured stock RAPD genetic diversity |
