| 摘要: |
| 对采自广西涠州岛近海多管藻(Polysiphonia urceolata)中分离到的一株内生真菌EN-22的次生代谢产物的化学成分进行了研究。利用正相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析、制备薄层层析(PTLC)以及重结晶等分离手段从该菌发酵液中分离得到15个化合物，通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构，分别为：4-(4-喹啉)-4-羟基-2-丁酮(1)，3-羟基-3-(2-氧丙基)吲哚-2-酮(2)，3-吲哚乙醇(3)，3-吲哚甲酸(4)，2-羟基-3-吲哚丙酸(5)，2-酮-3-吲哚乙醇(6)，3,3-二吲哚-2-羟基-丙醇(7)，β-咔啉(8)，尿嘧啶(9)，环-(S-脯氨酸-S-异亮氨酸)(10)，(22E,24R)-3β,5α,9α-三羟基麦角甾-7,22-二烯-6-酮(11)，(22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7α-二醇(12)，5α,6α-环氧-24(R)-甲基胆甾-7,22-二烯-3β-醇(13)，胡萝卜苷(14)，过氧化麦角甾醇(15)；其中化合物1和2为新天然产物，并首次报道化合物1的碳谱数据。
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| 关键词: 多管藻(Polysiphonia urceolata) 内生真菌 化学成分 结构鉴定 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30530080）；山东省科技发展计划项目（2006GG2205023）；中国科学院院长基金资助项目 |
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| Chemical constituents of EN-22, an endophytic fungus derived from the marine red alga Polysiphonia urceolata |
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ZHAO Ting-ting,LI Xiao-ming,LI Jing-mei,LI Ke,CUI Chuan-ming,LI Chun-shun,WANG Bin-gui
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| Abstract: |
| From the fermentation culture of an endophytic fungus EN-22 that was derived from the marine red alga Polysiphonia urceolata, fifteen compounds were isolated by column chromatography separation on the normal phase silica gel and Sephadex LH-20, and by the preparative thin layer chromatography (PTLC) as well as by re-crystallization. Structures of all purified compounds were elucidated by analysis of their spectroscopic data including MS, 1D and 2D NMR to be 4-(4-Quinolyl)-4 hydroxy-2-butanone (1) , 3-hydroxy-3-(2-oxopropyl)indo-lin-2-on (2), 2-(1H-indol-3-yl)ethanol (3), 1H-indole-3-carboxylic acid (4), 2-hydroxy-3-(1H-indol-3-yl) propanoic acid (5), 2-hydroxy-1-(1H-indol-3-yl)ethanone (6), 3,3-di(1H-indol-3-yl)propane-1,2-diol (7), β-carbolines (8), uracil (9), cyclo-(S-Pro-S-Ile) (10), (22E,24R)-3β,5α,9α-trihydroxy-ergosta-7,22-diene-6-one (11), 22E,24R-5α,6α-epoxyergosta-8,22-dien-3β,7α-diol (12), 5α,6α-epoxy-24(/i>R)-methylcholesta-7,22-dien-3β-ol (13), daucosterol(14), and ergosterol peroxide (15). Compounds 1 and 2 were reported as new natural products
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| Key words: Polysiphonia urceolata Endophytic fungus EN-22 chemical constituents structure elucidation |
