引用本文:	刘晨临,黄晓航,刘建国.4株形态不同的长心卡帕藻（Kappaphycus alvarezii）的初步ISSR分析[J].海洋科学,2009,33(4):50-53.

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*4株形态不同的长心卡帕藻（Kappaphycus alvarezii）的初步ISSR分析*
刘晨临¹, 黄晓航¹, 刘建国²
1.国家海洋局第一海洋研究所;2.中国科学院海洋研究所

摘要:

为了研究高产胶量品系的遗传学特征，建立了长心卡帕藻(Kappaphycus alvarezii)物种的ISSR分析方法。用ISSR技术对4株同一来源，形态各异的长心卡帕藻进行了遗传学分析，与11条ISSR引物共扩增得到60条带，其中1条带具有多态性并推测与稳定的经济性状连锁。说明ISSR技术可应用于长心卡帕藻品系筛选，结合其他手段，为分子标记辅助育种提供了可能。

关键词: 长心卡帕藻(Kappaphycus alvarezii) ISSR 种质鉴定

DOI：

分类号:

基金项目:国家海洋公益项目（200705010）

The primary application of ISSR analysis on strains identification of Kappaphycus alvarezii

Abstract:

To investigate the genetic properties of high Carrangeenans production strains, the method of ISSR analysis of Kappaphycus alvarezii was established. The genetic diversities among 4 strains of K. alvarezii of the same origin which differs in configuration were analyzed. A total of 60 amplification loci were obtained from 11 ISSR primers, of which 1 locus revealed a polymorphism and possible linkage to the economic properties. It was indicated that the feasibility of ISSR could be combined with other measures on K. alvarezii.

Key words: Kappaphycus alvarezii inter-simple sequence repeat strains of selection