| 摘要: |
| 以海藻废弃物堆肥中分离到的薄壁杆菌属(Gracillibacillus)的褐藻酸降解菌A7为研究对象, 对该菌的生长及产酶条件进行了研究。结果表明, 该菌的最适产酶条件为: 温度30℃, 海藻酸钠的质量分数0.5%,pH9.5,NaCl浓度0.5mol/L, 以蛋白胨为主要氮源。在最佳条件下培养96 h达到最高酶活力12.79 U/mL。 |
| 关键词: 褐藻酸降解菌 褐藻酸裂解酶 产酶条件 薄壁杆菌属(Gracillibacillus) |
| DOI： |
| 分类号: |
| 基金项目:国家863计划重点资助项目( 2007AA061201) |
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| Study on culture conditions for alginase production by alginate degrading bacterium Gracillibacillus sp. A7 |
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| Abstract: |
| A bacterium A7 with relatively high alginase activity was isolated from wakame compost and belongs to genus Gracillibacillus. Based on the activity detection of the fermentation liquid, the optimum conditions of alginase production were determined as follows: pH9.5; temperature, 30℃; sodium alginate and sodium chloride, 0.5% and 0.5 mol/L, respectively; peptone as the principal source of nitrogen and culturing continuously for 72h. |
| Key words: alginate lyase alginase activity optimum conditio ns Gracillibacillus |
