| 摘要: |
| 采用免培养的16S rDNA梯度凝胶电泳技术(DGGE)对集约化海水网箱养殖川纹笛鲷(Lutjanus sebae)的消化道胃壁、胃内容物、肠壁、肠内容物优势菌群结构进行了比较分析。研究结果显示,川纹笛鲷消化道存在着丰富多样的细菌群落,对DGGE指纹图谱聚类分析表明菌群组成相似度高于55%,其中胃内容物及胃壁细菌组成相似度最高(90%),这可能与摄食饵料在消化道推移有关;而胃壁与肠壁相似度相对最差,可能反应了由于生理环境不同引起的宿主差异性。通过建立川纹笛鲷消化道16S rDNA2DGGE指纹图谱及比较分析,为澄清川纹笛鲷消化道微生物区系奠定了基础。 |
| 关键词: 川纹笛鲷(Lutjanus sebae) 16S rDNA 梯度凝胶电泳 指纹图谱 |
| DOI： |
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| 基金项目:国际科技合作重点项目(2004DF100229) ;中国博士后科学基金项目(2005037625) ;淡水生态与生物技术国家重点实验室开放课题(2005FB05) ;广东省自然科学基金项目(05300945) |
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| Comparison of the PCR-DGGE fingerprints in the gastrointestinal predominant bacterial community in Lutjanus sebae |
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| Abstract: |
| The comparison of the predominant bacterial community structure in the gastro-intestine in Lutjanus sebae based on 16S rDNA PCR-DGGE fingerprint using a culture-independent method was conducted in present research. The results showed that abundant bacterium existed in the gastro-intestine of Lutjanus sebae, the similarity was above 55% in the community structures in the gastro-intestine with the highest similarity of 90% in the bacteria ingredients between the stomach wall and the stomach content, which might reflect the diet feed processed from the stomach to the intestine. However, the difference of the relative abundance between the stomach wall and the intestinal wall in the bacterium might be due to their different physical environments. The establishment and comparison of the 16S rDNA-DGGE fingerprint in the gastro-intestine of the finfish in present research do help to elucidate the microflora structure in the gastro-intestine in Lutjanus sebae. |
| Key words: Lutjanus sebae 16S rDNA DGGE fingerprint |
