引用本文:	刘　杨,王雪青,庞广昌,谢卓峰.双水相萃取法富集分离螺旋藻藻蓝蛋白的研究[J].海洋科学,2008,32(7):30-32.

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双水相萃取法富集分离螺旋藻藻蓝蛋白的研究
刘　杨¹, 王雪青¹, 庞广昌¹, 谢卓峰¹
天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院

摘要:

以PEG/硫酸钠双水相体系,经一次萃取从钝顶螺旋藻(Spirulina platensis)细胞破碎液中富集分离藻蓝蛋白。结果表明,萃取最适宜的条件为12% PEG4000,15% Na₂SO₄,1% KCl,藻蓝蛋白收率为91.2%,分配系数达到8.01,分离因数达到6.33。对于螺旋藻藻蓝蛋白的富集分离,双水相萃取法与传统的盐析沉淀法相比,具有节省操作时间、简化操作过程、降低能耗和成本以及易于工艺放大等优点。

关键词: 螺旋藻(Spirulina) 藻蓝蛋白聚乙二醇双水相体系萃取

DOI：

分类号:

基金项目:天津市重大科技攻关项目(06YFGZNC04200)

Aqueous two-phase extraction for enrichment and isolation of c-phycocyanin from Spirulina

Abstract:

Aqueous two-phase system composed of PEG/ Na₂SO₄ was once to enrich and isolate c-phycocyanin from the supernatant of disrupted Spirulina cell in this paper. The optimum extraction conditions were: 12% PEG, 15% Na₂SO₄, 1% KCl, the partition coefficient of c-phycocyanin was as high as 8.01, with a yield of 91.2%. The separation factor was increased to 6.33. The preliminary study indicated that aqueous two-phase extraction was better than traditional salt-out precipitation for enrichment and isolation of c-phycocyanin.

Key words: Spirulina c-phycocyanin polyethylene glycol (PEG) aqueous two-phase system extraction