| 摘要: |
| 应用胃泌素多克隆抗体和链霉菌抗生物素蛋白-过氧化物酶免疫 组织化学方法(SP法),对中华乌塘鳢(Bostrichthys sinensis)、黄鳍鲷(Sparus latus)、牙鲆(Paralichthys olivaceus)、褐篮子鱼(Sigauns fuscesens)和大弹涂鱼(Boleophthalmus pectinirostris)消化道胃泌素细胞(G细胞)进行免疫组化鉴别和定位。结果显示:中华乌塘鳢G细胞主要分布在小肠,直肠偶见。黄鳍鲷G细胞 仅在小肠发现。牙鲆G细胞大量位于幽门胃和小肠。褐篮子鱼G细胞在幽门盲囊和小肠有少量分布。大弹涂鱼G细胞在贲门胃、幽门胃和小肠均有出现,大量分布于 幽门胃。5种鱼类G细胞主要出现在胃和小肠的分布状况,与胃泌素调节胃肠消化吸收的功能相一致。5种鱼类G细胞形态类型多样,提示了胃泌素以不同的内分泌 方式参与消化生理过程。 |
| 关键词: 海洋鱼类 消化道 胃泌素细胞 免疫组织化学 |
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| 基金项目:国家自然科学基金资助项目( 40276040) ; 福建省自然科学基金资助项目( B0210003) |
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| The localization of G cell in the digestive tract in five kinds of marine fish |
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| Abstract: |
| By using the gastrin antiserum and the immunohistochemical method of Streotavidin-Peroxid(S-P), the investigation on the distributions of G cells in the digestive tract of Bostrichthys sinensis, Sparus latus, Paralichthys olivaceus, Sigauns fuscesens and Boleophthalmus pectinirostris was made. The distributions of the G cells are as follows: In Bostrichthys sinensis, the G cells are mainly located in intestine, rarely in rectum; In Sparus latus, the G cells were only observed in intestine, none in any other parts of the digestive tract; In Paralichthys olivaceus, the G cells were observed numerously in stamachus pyloricus and intestine; In Sigauns fuscesens, the G cells were distributed in pyloric caecum and intestine; In Boleophthalmus pectinirostris, the G cells were observed in stomachus carsiacus, stamachus pyloricus and intestine,mainly located in stamachus pyloricus. The G cells were detected mainly in the stomach and intestine in 5 kinds of marine fish, the results show that the gastrin was correlated with function of digestion and absorption. The G cells were of polymorphism in 5 kinds of marine fish. It was suggested that the gastrin might exert the digestive physiology in different modes of endocrine. |
| Key words: mrine fish digestive tract gastrin cell immunohistochemistry |
