摘要: |
采用MTT法(四甲基偶氮唑盐比色法)研究了钝顶螺旋藻多糖 (PSP)对Hela细胞及HepG2细胞生长的抑制作用。结果表明,随PSP浓度及培养时间的增加,肿瘤细胞存活率逐渐降低,抑制率逐渐增加,以 PSP40 mg/L作用72 h最为显著。应用Annexin V/PI双染色流式细胞仪检测了早期Hela细胞凋亡,未经PSP处理的正常Hela细胞凋亡细胞极少,经PSP处理的细胞,凋亡细胞的百分比明显高于正 常对照组,其作用随着剂量的增加和时间的延长而增强,具有量效关系和时效关系。结果说明PSP的抗肿瘤机制,除了诱导肿瘤细胞凋亡,还存在细胞毒性等其他 机制,是多种机制共同作用的结果。 |
关键词: 钝顶螺旋藻多糖 抗肿瘤作用 细胞凋亡 AnnexinV/PI |
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Experimental studies on antiproliferation activities and early apoptosis inducement of polysaccharides from Spirulina platensis |
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Abstract: |
In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay, the results showed that PSP(2.5~40 mg/L, 48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining, the apoptosis rates induced by PSP(5, 10, 20, 40 mg/L) were 30.3%, 32.6%, 34.8% and 38.8% respectively, which were higher than those of the normal control group(P<0.05). Meanwhile, the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner. These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis. |
Key words: polysaccharides from Spirulina platensis(PSP) antitumor effect apoptosis annexin V/PI |