| 摘要: |
| 以羟基磷灰石柱层析法从小珊瑚藻(Corallina pilulifera)中提取出藻红蛋白,其纯度可达A565/A280大于3,得率为0.173g/kg。该藻红蛋白在498nm和565nm处有两处荧光激发峰,为双峰型。荧光发射光谱检测表明藻红蛋白在pH5.0～10.0溶液中具有较高的稳定性,其中以pH6.0和pH10.0的稳定性最高。该藻红蛋白对光照敏感,光照度800lx照射17h后荧光基本消失。对氧化剂(H2O2)敏感,在9℃以0.1%的H2O2处理24h,荧光基本消失。藻红蛋白不耐高温,80℃处理0.5h,导致蛋白液褪色,荧光消失。 |
| 关键词: 小珊瑚藻(Corallina pilulifera) 藻红蛋白 荧光发射光谱 稳定性 |
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| Extraction and stability study of phycoerythrins of Corallina pilulifera |
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| Abstract: |
| The phycoerythrin of Corallina pilulifera has been extracted as the purity more than 3.0 by the ratio of A565/A280 by hydroxylapatite column chromatography. 0.173 g phycoerythrin could be obtained from every 1 kg fresh C. pilulifera. The phycoerythrin showed two fluorescence emission apexes, ie, 498 nm and 565 nm. So it belongs to the double-apexe type of phycoerythrin. Its fluorescence emission spectra showed that the phycoerythrin was high stable in the solution with pH ranged from 5.0～10.0, and that the two high stable points of phycoerythrin appeared at pH 6.0 and pH 10.0 respectively. It was sensitive to light. The fluorescence was generally disappeared after exposing under 800 lx for 17 h. It was also sensitive to oxidants, which make fluorescence disappeare after exposing to H2O2(0.1 %) under 9 ℃ for 24 h. The color of the phycoerythrin could be faded and its fluorescence losed when it was treated under high temperature 80 ℃ for 0.5 h. |
| Key words: Corallina pilulifera phycoerythrin fluorescence emission spectra stability |
