| 摘要: |
| 取红鳍笛鲷(Lutjanus erythopterus Bloch)、紫红笛鲷(Lutjanus argentimaculatus Forskal)、勒氏笛鲷(Lutjanus russelli Bleeker)和约氏笛鲷(Lutjanus johni Bloch)肝脏组织,分离纯化其线粒体DNA,用限制性内切酶酶切进行了限制性片段长度多态性分析。在红鳍笛鲷共发现4种单倍型,单倍型间的平均遗传距离为0.010 1,其mtDNA多态度为0.002 9。在紫红笛鲷共发现6种单倍型,单倍型间的平均遗传距离为0.015 1,其mtDNA多态度为0.005 5。在勒氏笛鲷共发现3种单倍型,单倍型间的平均遗传距离为0.008 6,其mtDNA多态度为0.001 2。在约氏笛鲷共发现5种单倍型,单倍型间的平均遗传距离为0.008 3,其mtDNA多态度为0.002 0。 |
| 关键词: 笛鲷 遗传多样性 mtDNA |
| DOI： |
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| 基金项目:广东省科技计划项目重大专项(A3050202);湛江市科技攻关项目(200017) |
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| Diversity analysis of mtDNA in the four species of snappers(Lutjanus) |
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| Abstract: |
| We used mtDNA-RFLP technique to analyse the genetic diversity of 4 species of snappers, Lutjanus erythopterus, Lutjanus argentimaculatus, Lutjanus russelli and Lutjanu johni. The mitochondrial DNAs(mtDNAs) from livers of these fishes were isolated by means of differential centrifugation and nuclease digestion followed by phenol extraction. The mtDNAs were digested by 19 varieties of restriction enzymes(((5 or 6)) base pairs): BamH I, Bgl I, Bgl II, EcoR I, EcoR V, Hind III, Hinf I, Hpa I, Kpn I, Mlu I, Pst I, Pvu II, Sal I, Sca I, Sma I, Sty I, Xba I and Xho I, analysed by using agarose gel electrophoresis and detected by Gel-Pro Analyzer. Restriction enzyme maps revealed that 4 kinds of haplotypes exsited in L. erythopterus, 3 in L .russelli, 6 in L. argentimaculatus and 5 in L. johni. We also discussed the mechanism of this phenomenon based on restriction sites variant, calculated the nucleotide diversity of inter-species genetic distance. The polymorphic degrees of mtDNAs from above four snappers were 0.002 9, 0.005 5, 0.001 2 and(0.002 0) respectively. |
| Key words: Lutjanus genetic diversity mtDNA |
