引用本文:	陈骁,左正宏,姚继承,陈奕欣.几种紫菜种质资源遗传多样性的RAPD分析[J].海洋科学,2005,29(4):76-81.

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几种紫菜种质资源遗传多样性的RAPD分析
陈骁¹, 左正宏¹, 姚继承¹, 陈奕欣¹
厦门大学生命科学学院

摘要:

用随机多态DNA(RAPD)标记方法对11个紫菜(Porphyra)样品进行遗传多样性检测,从46个随机引物中筛选出27个有效引物,共扩增出282条DNA带,其中多态位点224个,占79.4%。用类间平均链锁法对各样品间的遗传距离进行聚类分析。结果显示,2个条斑紫菜之间的亲缘关系很近,遗传相似系数达98.1%,和坛紫菜的亲缘关系较远;坛紫菜不同样品间的遗传相似系数在75.8%~90.6%之间,表明坛紫菜种质资源具有丰富的遗传多样性,是良种选育的遗传基础。

关键词: 随机多态DNA(RAPD) 紫菜(Porphyra) 遗传多样性

DOI：

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基金项目:国家863计划项目(2002A603023);福建省重大农业科技项目(2001Z017)

Analysis of genetic diversity of Porphyra by random amplified polimorphic DNA

Abstract:

The genetic diversity of 11 Porphyra samples were confirmed by random amplified polimorphic DNA(RAPD) markers. 27 primers were screened from 46 arbitrary primers, and a total of 282 DNA bands were amplified, among them 79.4% were polymorphic. The genetic data were analyzed by means of similarity, in a dendrogram was using between groups linkage method. The result showed that a close relationship existed between two cultivears of Porphyra yezoensis and the genetic coefficient comparability was 98.1%, while the genetic diversity level in Porphyra haitanesis was relatively high.

Key words: random amplified polimorphic DNA(RAPD) Porphyra genetic diversity