| 摘要: |
| 通过培养褐藻酸降解菌交替单胞菌(Alteromonas sp.)菌株H -1使其产酶,研究了该酶的性质。结果表明 ,该菌在25℃培养72h时产酶量最高。褐藻酸酶作用的最适底物质量分数为1%～2% ,最适pH值为7.5 ,最适反应温度为40℃ ,温度升高酶活力急剧下降。 |
| 关键词: 褐藻酸降解菌 褐藻酸降解酶 制备 |
| DOI： |
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| 基金项目:国家海洋863资助项目 (D12022509,D10022508) |
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| Preparation of alginase and its properties |
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| Abstract: |
| Alginase was prepared by culturing an alginic acid decomposing bacterium Alteromonas sp. strain H - 1. The bacterium produced a maximum amount of alginase when it was incubated at 25 ℃ for 72h. The optimum alginate sodium concentration was 1% ～ 2%, the optimum pH and temperature was 7.5 (Tris - HCl buffer) and 40 ℃ respectively. The activity of the enzyme would reduce sharply if the temperature was above 40 ℃. |
| Key words: alginic acid decomposing bacterium alginase preparation property |
