| 摘要: |
| 对分离自腌鱼的高产菌红素 (bacterioruberin)的菌株r-fish进行了形态特征、生理生化特性与细胞化学组分的分析以及基于16SrDNA序列的系统发育分析。形态特征、生理生化特性及细胞化学组分的分析结果表明菌株r-fish为球形的嗜盐古菌。基于16SrDNA序列的系统发育结果表明 ,r-fish与HalobacteriumtrapanicumNCIMB784菌株的相似性为99.8% ,归入Natrinema属。用TLC法对r -fish的色素进行了分析 ,r -fish的色素组分包括菌红素(bacterioruberin)、脱水菌红素 (anhydrobacterioruberin)、双脱水菌红素 (bisanhydrobacterioruberin)和一未知色素。与Halobacterium halobium和Halococcus morrhuae的色素组分相似。 |
| 关键词: 菌红素(bacterioruberin) 菌红素产生菌 嗜盐古菌 16SrDNA序列,系统发育树 |
| DOI： |
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| 基金项目:江苏大学青年自然科学基金资助项目(1241180004) |
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| Identification of a bacterioruberin-producing microorganism and analysis of its pigment |
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| Abstract: |
| A strain r - fish that produces red pigments was isolated from salted fish. Based on its morphological, cultural, physiological and biochemical characteristics, strain r - fish is certainly an coccoid extremely halophilic archaeon. Phylogenetic analysis results indicate that r - fish and Halobacterium trapanicum NCIMB 784 both belong to the genus of Natrinema at the similarity of 99. 8%. The red pigments of r - fish were isolated and subjected to TLC analysis. The red pigments of r - fish contained, bacterioruberin, anhydrobacterioruberin, bisanhydrobacterioruberin and an unidentified pigment, similar to the pigment compositions of Halobacterium halobium and Halococcus morrhuae. |
| Key words: bacterioruberin bacterioruberin - producing microorganism halophilic archaeon 16S rDNA sequence phylogenetic tree |
