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| A QUICK ASSAY FOR TETRACYCLINE |
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| Abstract: |
| An anti-luminescent assay of Tetracycline that, like many antibiotic substances, quenches the light of marine form luminous bacteria effectively was studied with Photobacterium phosphoreum A2 as an indicator. Good results were obtained in tests with luminous bacterial concentration ca. 5×107 cells/ml. Light intensity was determined by photomultiplier (GDBIOM) of photoluminometer. Experimental data were expressed as voltage or a percentage of a control. Within the range of 30 μ/ml to 140μ/ml, the curves of tetracycline concentration plotted against light intensity gave good linearity. The correlation coefficient of the curves obtained were -0.996, -0.978, -0.988. The tests could be done within an hour. So, comparing with cylinder plate assay, this one is faster and simpler. |
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