| 摘要: |
| 弹涂鱼是广泛分布于西北太平洋地区的一种两栖性鱼类的统称，具有重要的经济价值和生态价值。研究基于线粒体ND5基因序列比较分析了西北太平洋地区4种弹涂鱼，即弹涂鱼(Periophthalmus modestus)、大鳍弹涂鱼(Periophthalmus magnuspinnatus)、大弹涂鱼(Boleophthalmus pectinirostris)、薄氏大弹涂鱼(Boleophthalmus boddarti)的群体遗传多样性和遗传结构。研究结果显示，在长度549bp的线粒体ND5序列中，单倍型多样度:弹涂鱼>薄氏大弹涂鱼>大弹涂鱼>大鳍弹涂鱼，核苷酸多样度:大弹涂鱼>弹涂鱼>薄氏大弹涂鱼>大鳍弹涂鱼。此外，基于单倍型序列构建的邻接关系树可以看出，4种弹涂鱼之间的单倍型可各自聚成单系，但种内不存在显著的谱系结构。群体间的遗传分化指数(Fst)表明弹涂鱼中日群体间存在显著分化，大弹涂鱼湛江、厦门、温州群体两两之间遗传分化与其地理距离成正比。分子方差分析(AMOVA)的结果显示分子变异主要来自于群体内，说明其种群内部整体分化程度不高。中性检验、核苷酸不配对分布以及贝叶斯天际线分析结果均表明弹涂鱼和大弹涂鱼在更新世末期可能经历了群体扩张事件。该研究对西北太平洋海域的4种弹涂鱼的遗传变异进行了全面的比较分析，研究结果可为弹涂鱼鱼类的资源保护与合理利用提供基础资料。 |
| 关键词: 西北太平洋 4种弹涂鱼类 遗传多样性 遗传结构 |
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| GENETIC DIVERSITY OF FOUR MUDSKIPPER (GOBIIDAE) IN THE PACIFIC NORTHWEST BASED ON MTDNA ND5 GENES |
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Xiao Dongdong1, WU Zengman1, Zhao Linlin2, Song Na1
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1.Fisheries College, Ocean University of China, Qingdao;2.The First Institute of Oceanography, MNR, Qingdao
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| Abstract: |
| Mudskippers are a collective term for amphibious fish widely distributed in the Northwest Pacific, possessing significant economic and ecological value. This study conducted a comparative analysis of the population genetic diversity and genetic structure of four mudskipper species in the Northwest Pacific, namely, Periophthalmus modestus, Periophthalmus magnuspinnatus, Boleophthalmus pectinirostris, and Boleophthalmus boddarti, based on the mitochondrial ND5 gene sequence. The research results found that, in the 549bp mitochondrial ND5 sequence, the haplotype diversity of P. modestus was the highest, followed by B. boddarti, B. pectinirostris and P. magnuspinnatus. The nucleotide diversity of B. pectinirostris was the highest, followed by P. modestus, B. boddarti and P. magnuspinnatus. The constructed neighbor-joining tree based on haplotypes showed that the above four mudskipper species were clustered into separate clades, but there was no significant phylogenetic structure within each species. The genetic differentiation index (Fst) results of P. modestus suggested significant differentiation between the Chinese and Japanese populations. For B. pectinirostris, Fst between populations Zhanjiang, Xiamen, and Wenzhou was proportional to geographical distance. Analysis of Molecular Variance (AMOVA) indicated that molecular variation mainly came from within populations, suggesting a relatively low level of differentiation within populations. Neutral tests, nucleotide mismatch distribution, and Bayesian skyline plot analysis all suggested that mudskipper and B. pectinirostris might have experienced a population expansion event at the end of the Pleistocene. Overall, this study provided a comprehensively comparative analysis of genetic variation of four mudskipper species in the Northwest Pacific, offering fundamental data for their resource conservation and rational utilization. |
| Key words: Northwestern Pacific four mudskipper species Genetic diversity Genetic structure |