| 摘要: |
| 酪蛋白激酶1 (CK1)是一种重要的蛋白激酶家族, 其在DNA损伤应答和修复、细胞增殖和凋亡、胚胎发育和稳态等重要的生物学过程中都具有复杂多样的调控作用。为了理解CK1基因家族在双壳贝类中的特征、进化及生物学功能, 实验采用比较基因组学及生物信息学的方法对双壳贝类CK1基因家族进行了鉴定分析, 通过虾夷扇贝高温应激实验, 研究了CK1基因家族在高温应激时的表达规律。结果显示, 在虾夷扇贝、栉孔扇贝、长牡蛎与侏儒蛤中均存在CK1α, CK1αlike, CK1δ, CK1γ3,并发现CK1ε, CK1γ1, CK1γ2在双壳贝类中发生了丢失。时空表达分析发现, 双壳贝类CK1基因均在胚胎发育早期集中表达, 并以多细胞/囊胚期为界呈现出母源/合子特异性表达模式。CK1为关键基因的基因共表达模块显著富集在错配修复、核苷酸剪切修复等相关通路上, 暗示了CK1基因在双壳贝类胚胎发育过程中参与调控DNA损伤修复过程, 从而维持早期胚胎发育时的基因组稳定性。双壳贝类CK1基因在成体中呈现组织特异的表达模式, 主要在鳃和雄性性腺中具有相对较高的表达量。虾夷扇贝受到高温应激后, 其鳃中PyCK1α, PyCK1αlike和PyCK1δ在前3 h显著上调, 但在12 h后趋于下调, 而PyCK1γ3在高温应激不同时间点均表现出下降趋势, 表明双壳贝类CK1基因参与响应高温应激反应。此项研究有助于理解双壳贝类CK1基因的功能和进化, 为解析双壳贝类胚胎发育稳态维持和应激调节机制提供理论基础。 |
| 关键词: 双壳贝类 CK1基因家族 基因鉴定 表达分析 高温应激 |
| DOI：10.11693/hyhz20221200336 |
| 分类号:Q38 |
| 基金项目:国家重点研发计划项目,2022YFD2400301号;山东省重点研发计划项目,2021ZLGX03号。 |
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| GENOME-WIDE IDENTIFICATION AND EXPRESSION PROFILING OF THE CK1 GENE FAMILY IN BIVALVES |
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SUN Feng-Zhi1, LYU Zhen-Li1, LIU Fu-Yun1, XING Qiang1, LIAN Shan-Shan1, WANG Shi1,2,3, BAO Li-Sui4
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1.Ministry of Education (MOE) Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China;2.Laboratory for Marine Biology and Biotechnology, Laoshan Laboratory, Qingdao 266237, China;3.Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Ocean University of China, Sanya 572000, China;4.Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| The casein kinase 1 (CK1) gene family is an important family of protein kinases with complex and diverse regulatory roles in important biological processes such as DNA damage response and repair, cell proliferation and apoptosis, and embryonic development and homeostasis. To understand the characteristics, evolution, and biological function of the CK1 gene family in bivalve species, we conducted the genome-wide identification and functional and evolutionary analysis of the CK1 gene family in bivalve. We also investigated the expression profiling of the CK1 gene family under heat stress. The genome-wide characterization revealed that CK1α, CK1αlike, CK1δ, and CK1γ3 were present and CK1ε, CK1γ1, and CK1γ2 were found lost in P. yessoensis, C. farreri, C. gigas, and M. lateralis. Spatiotemporal expression analysis revealed that all CK1 genes in bivalve were expressed in mainly the early embryonic development and showed a maternal/zygotic specific expression pattern at the multicellular/blastula stage; and the co-expression module of CK1 as a key gene was significantly enriched in mismatch repair, nucleotide excision repair, and other related pathways, suggesting that CK1 genes are potentially involved in regulating DNA damage repair during bivalve embryonic development, thus to maintain the genomic integrity during embryonic development. The CK1 genes in bivalve showed different expression patterns in adult tissues, mainly in gill and male gonad with relatively high expression. After heat stress on P. yessoensis, PyCK1α, PyCK1αlike, and PyCK1δ were significantly up-regulated in the first 3 h and then tended to be down-regulated after 12 h, while PyCK1γ3 showed a different downward trend at different time points of heat stress. These results indicate that bivalve CK1 genes are involved in the response to heat stress. This study contributes to the understanding of the function and evolution of bivalve CK1 genes and lays a foundation for further investigating the mechanisms of homeostasis maintenance during embryonic development and stress regulation in bivalve species. |
| Key words: bivalve CK1 gene family gene identification expression analysis heat stress |
