| 摘要: |
| 雨生红球藻(Haematococcus pluvialis)是天然虾青素的最佳来源, 它可以在高光胁迫等不利条件下转变成厚壁细胞并积累大量虾青素。然而, 目前从微小RNA (microRNA, miRNA)层面来揭示在高光胁迫下雨生红球藻次级细胞壁形成和虾青素合成的机理研究尚未见报道。对高光处理下三个时间点的雨生红球藻样品进行microRNA测序, 共鉴定出342个已知miRNA和283个新miRNA。在这625个miRNA中, 其中有206个miRNA的表达量受高光影响, 这些差异表达的miRNA的靶基因参与了淀粉与蔗糖代谢、甘露糖与果糖代谢、糖酵解和萜类骨架生物合成等重要代谢通路。结合转录组结果发现, 高光胁迫下有6个miRNA调控了3个和次级细胞壁形成相关靶基因的表达, 有5个miRNA调控了5个和虾青素生物合成相关靶基因的表达。以上结果揭示了雨生红球藻在高光下miRNA调控次级细胞壁形成和虾青素生物合成的潜在机制, 为雨生红球藻虾青素的代谢工程奠定了理论基础。 |
| 关键词: 雨生红球藻 microRNA 次级细胞壁虾青素 高光胁迫 |
| DOI：10.11693/hyhz20220800205 |
| 分类号:Q946; Q789; S968.4 |
| 基金项目:国家自然科学基金项目,31572638号 |
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| THE MECHANISM UNDERLYING MICRORNA-MEDIATED SECONDARY CELL WALL FORMATION AND ASTAXANTHIN ACCUMULATION UNDER HIGH LIGHT STRESS IN HAEMATOCOCCUS PLUVIALIS |
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CHEN Feng1,2, HU Chao-Yang1,2, SUN Xue1,2, XU Nian-Jun1,2
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1.School of Marine Science, Ningbo University, Ningbo 315211, China;2.Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China
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| Abstract: |
| Haematococcus pluvialis is the best source of natural astaxanthin, can transform into thick-walled cells for accumulating large amounts of astaxanthin under unfavorable conditions such as high light. However, there are lack of studies at microRNA (miRNA) level on the mechanism of secondary cell wall formation and astaxanthin biosynthesis under high light stress in H. pluvialis. In this study, microRNA sequencing was performed in H. pluvialis treated with high light at three time points. A total of 342 known miRNAs and 283 novel miRNAs were identified, among which 206 miRNAs were differentially expressed under high light. The target genes of these differentially expressed miRNAs (DEmiRNA) were involved in important metabolic pathways such as starch and sucrose metabolism, mannose and fructose metabolism, and glycolysis and terpene backbone biosynthesis. We found that combined with the transcriptome results, six miRNAs regulated the expression of three target genes that related to the secondary cell wall formation, and five miRNAs regulated the expression of five target genes that related to astaxanthin biosynthesis under high light stress. These results revealed the underlying mechanism of miRNA regulation of the secondary cell wall formation and astaxanthin biosynthesis under high light in H. pluvialis, and laid a theoretic foundation for metabolic engineering of astaxanthin in H. pluvialis. |
| Key words: Haematococcus pluvialis microRNA secondary cell wall astaxanthin high light stress |
