| 摘要: |
| 为优化大黄鱼（Larimichthys crocea）麻醉运输技术，提高保活率，以不同浓度MS-222麻醉剂对其运输存活率、呼吸代谢、鱼体生理生化指标的影响展开研究。结果显示：（1）12 h模拟运输后，10 mg/L麻醉组大黄鱼存活率显著高于其他实验组（P<0.05），30 mg/L麻醉组积累死亡率已高于50%；（2）呼吸频率随麻醉剂浓度的升高显著降低（P<0.05），各组水体溶解氧含量随运输时长增加均呈下降趋势；（3）10和20 mg/L麻醉组大黄鱼复苏率分别为89%±10%和63%±19%，且均显著高于30 mg/L麻醉组（P<0.05）；（4）随麻醉剂浓度的升高，大黄鱼血清中皮质醇、谷草转氨酶、乳酸脱氢酶、葡萄糖-6-磷酸酶水平逐渐降低，其肌肉乳酸水平显著降低（P<0.05），其肌糖原水平显著升高（P<0.05）。结果表明：高浓度（30 mg/L） MS-222处理会引起大黄鱼呼吸抑制，造成运输存活率下降，不适合应用于大黄鱼的保活运输；低浓度（10 mg/L） MS-222处理可以达到降低鱼体拥挤胁迫、应激反应及呼吸代谢的效果，并可以有效降低大黄鱼死亡率，保活时间可达12 h。 |
| 关键词: 大黄鱼(Larimichthys crocea) 保活运输 麻醉剂 应激反应 |
| DOI：10.11693/hyhz20220600171 |
| 分类号:S981.14 |
| 基金项目:国家重点研发计划项目，2019YFD0900901号。 |
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| EFFECT OF ANESTHETIC TRANSPORTATION OF LARIMICHTHYS CROCEA IN MS-222 |
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ZHANG Wei-Jia, LI Hai-Dong, GAO Yang, CHU Zhang-Jie
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School of Fisheries of Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| To optimize the anesthesia transportation technology and improve the survival rate of Larimichthys crocea, the effects of different concentrations of MS-222 anesthetics on the survival rate and respiratory metabolism, and physiological and biochemical indices of L. crocea were studied. Results show that after 12 h simulated transportation, the survival rate of 10 mg/L anesthesia group was the highest, which was significantly higher than that of other experimental groups (P<0.05), and the cumulative mortality of 30 mg/L anesthesia group was higher than 50%. With the increase of the MS-222 concentration, the respiratory rate decreased significantly (P<0.05), and the dissolved oxygen content in each group showed a decreasing trend when transportation time increased. The recovery rates of L. crocea in 10 and 20 mg/L anesthesia groups were 89%±10% and 63%±19%, respectively, and that in the 30 mg/L anesthesia group was significantly lower than that in other two anesthesia groups (P<0.05). With the increasing anesthetic concentration in different groups, the levels of cortisol, aspartate aminotransferase, lactate dehydrogenase, and glucose-6-phosphatase in serum of L. crocea gradually decreased after transportation. With the increase of the concentration MS-222, the muscle lactic acid content decreased significantly (P<0.05), but the muscle glycogen content increased significantly (P<0.05). Therefore, the high concentration of MS-222 (30 mg/L) could inhibit the respiratory function of L. crocea, thus causing the decrease of the survival rate, which is not suitable for transportation of L. crocea. However, the low concentration of MS-222 (10 mg/L) could reduce the crowding stress, lower the stress response, and promote the respiratory metabolism, and eventually, increase the survival rate of L. crocea and extend the survival time up to 12 hours. |
| Key words: Larimichthys crocea live transport MS-222 stress response |
