| 摘要: |
| 本研究通过石蜡切片,苏木精-伊红染色对红鳍笛鲷(Lutjanus erythopterus)卵巢的发育进行了组织学研究,以期了解红鳍笛鲷的繁殖特性,为其人工繁殖和育苗提供参考资料。研究结果表明:红鳍笛鲷卵巢发育到生长成熟的过程,即卵巢从第1期发育到第4期,其生殖细胞可分为Ⅰ时相(卵原细胞),Ⅱ时相、Ⅲ时相、Ⅳ时相卵母细胞,在第2、3、4期卵巢中都发现有退化的卵母细胞形成的闭锁滤泡。4-6月龄卵巢分化完全,卵巢腔形成。卵原细胞从分散排列到成团排列在蓄卵板靠近卵巢腔一侧。8-25月龄的卵巢处于第2期,此时主要是Ⅱ时相的卵母细胞和卵原细胞,还有少数Ⅲ时相的卵母细胞。25月龄卵巢处于第3期,卵母细胞主要为Ⅲ时相和Ⅱ时相,也有少量的Ⅳ时相的卵母细胞,还有卵原细胞。2.5-3龄红鳍笛鲷卵巢可达4期,卵巢内多数为Ⅳ时相卵母细胞和Ⅲ时相卵母细胞,也有一定数量的Ⅱ时相卵母细胞和少数分散分布的卵原细胞。 |
| 关键词: 红鳍笛鲷 卵巢发育 卵母细胞 组织学 |
| DOI：10.11693/hyhz20151200306 |
| 分类号: |
| 基金项目:国家自然科学基金项目,31101904号;湛江市科技计划资助项目,2015B01074号;广东海洋大学创新强校项目,GDOU2013050307号。 |
|
| HISTOLOGY OF OVARIAN DEVELOPMENT OF CRIMSON SNAPPER LUTJANUS ERYTHOPTERUS |
|
LIU Hao,ZHANG Yu-hong,LUO Jie,LIU Chu-wu and LIU Li
|
|
Fisheries College,Guangdong Ocean University,Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes
|
| Abstract: |
| To understand the reproduction and provide reference for artificial propagation and breeding, histology of the ovary development of crimson snapper Lutjanus erythopterus was studied, for which paraffin section and hematoxylin-eosin staining were applied. The results show that during the ovary development to growth maturation, i.e., from the first stage to the fourth stage, germ cell development could be divided into 4 phases, named as the first (oogonia), second, third, and fourth. Degenerated oocytes were observed in all but the first stages. 4-6-month-old ovary differentiated completely with the formation of ovarian cavity, having oogonia and intersititial cells only. 8-25-month-old ovary contained mainly phase II oocytes and oogonia, with a few phase III oocytes. Ovary older than 25 months was said entering stage 3, in which phase Ⅲ oocytes dominated with some phase Ⅱ oocytes and oogonia, and a small amount of phase Ⅳ oocytes. 2.5-3 year-old ovary is defined reaching stage 4, in which most of oocytes were in phase Ⅳ and Ⅲ, and occasionally in phase Ⅱ; and a few oogonia scattered. |
| Key words: crimson snapper Lutjanus erythopterus ovarian development oocyte histology |
