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| 法螺(Chanoria tritonis)染色体核型分析 |
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蔡 岩,周永灿,冯永勤,吴开畅,谢珍玉,郭伟良,王世锋
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海南省热带生物资源可持续利用重点实验室 海南大学海洋学院 海口,海南省热带生物资源可持续利用重点实验室 海南大学海洋学院 海口,海南省热带生物资源可持续利用重点实验室 海南大学海洋学院 海口,中国水产科学研究院南海水产研究所 广州,海南省热带生物资源可持续利用重点实验室 海南大学海洋学院 海口,海南省热带生物资源可持续利用重点实验室 海南大学海洋学院 海口,海南省热带生物资源可持续利用重点实验室 海南大学海洋学院 海口
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| 摘要: |
| 法螺(Chanoria tritonis)是珊瑚敌害生物长棘海星的天敌, 在维持珊瑚礁生态系统平衡中具有重要作用。近年来由于人类过量捕杀, 法螺数量急剧减少, 开展法螺的人工繁育工作迫在眉睫。染色体研究是遗传育种工作的基础。目前, 有关法螺的染色体研究在世界范围内尚属空白。本实验以法螺面盘幼虫为材料, 采用秋水仙素溶液及低渗溶液处理, 热滴片法制片, 对染色体进行Giemsa染色来研究法螺的核型。结果表明, 法螺染色体二倍体数2n=70, 核型为 2n=32m+18sm+20st, NF=120。部分分裂相中出现2条异形染色体, 是否为性染色体还有待确认。染色体组中第2对和第16对中部着丝粒染色体上具有次缢痕。对我国已进行过染色体核型研究的腹足纲贝类进行统计表明, 法螺是目前已知的染色体数目最多的物种。 |
| 关键词: 法螺 染色体 核型 |
| DOI：10.11693/hyhz20130900125 |
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| 基金项目:国家自然科学基金项目, 41266004 号, 31060355 号; 国家海洋公益性项目, 201205025 号; 海南省重大科技项目,ZDZX2013009 号, ZDXM20110010 号; 海南省自然科学基金项目, 312069 号; 海南省教育厅高校科学研究资助项目, Hjkj2012-13号; 海南省产学研一体化专项资金, CXY20130028 号; 三亚市农业科技创新项目, 2012NK07 号 |
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| CHROMOSOME KARYOTYPE OF CHANORIA TRITONIS |
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Cai Yan,Zhou Yong Can,Feng Yong Qin,Wu Kai Chang,Xie Zhen Yu,Guo Wei Liang and Wang Shi Feng
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Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Marine Sciences Hainan University,Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Marine Sciences Hainan University,Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Marine Sciences Hainan University,South China Sea Fisheries Research Institute Chinese Academy ofFishery Sciences,Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Marine Sciences Hainan University,Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Marine Sciences Hainan University,Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Marine Sciences Hainan University
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| Abstract: |
| Feeding on thorn-starfish Acanthaster planci, the predator of coral polyps, Chanoria tritonis plays an important role in the coral reef ecosystem. However, its population has decreased dramatically during the last few decades. To help recover the natural C. tritonis population, breeding work on this snail is in urgent need. Although chromosome study is the foundation of breeding work, no chromosome study has been conducted on C. tritonis so far. In this study, using veliger larvae as experimental material, we obtained metaphase chromosomes of C. tritonis and decided the karyotype of C. tritonis. The results show that the karyotype of C. tritonis is 2n=32m+18sm+20st, NF=120. An idiosome was observed in certain proportion of metaphases. However, whether they are sex chromosomes remains unknown. Secondary constrictions were also found on individual chromosomes. With a diploid number of 70, C. tritonis has the largest number of chromosomes among all the Chinese gastropoda species studied. |
| Key words: Chanoria tritonis chromosome karyotype |