| 摘要: |
| 将银鲳幼鱼在盐度14、25、36条件下饲养96h,检测不同盐度组和不同时间点银鲳幼鱼鳃Na /K -ATP(NKA)酶活力、血清生长激素(GH)、胰岛素样生长因子Ⅰ(IGF-Ⅰ)和催乳素(PRL)浓度。结果显示,鳃NKA酶活力在三种盐度下总体呈先上升后恢复的变化,其最高点为14盐度48h时,且峰值显著高于其它值(P < 0.05)。36盐度组的血清GH浓度一直保持着较高水平,且与其它组均存在显著差异(P < 0.05),8h值为各组中最高值。14盐度组和36盐度组血清IGF-Ⅰ浓度都呈现先上升后恢复的变化。14盐度组血清催乳素浓度始终高于其它各盐度组,且在8h、24h和48h时显著高于其它盐度组(P < 0.05)。本试验发现：低盐度适应(14和25)时,血清GH浓度与NKA酶活力呈极显著正相关(P < 0.01)；高盐度适应(36和25)时,血清催乳素浓度与NKA酶活力呈显著负相关(P < 0.01)。另外,血清催乳素与IGF-Ⅰ浓度间有极显著正相关(P < 0.01)。研究证明银鲳幼鱼在适应不同盐度时,鳃NKA酶、生长激素、IGF-Ⅰ和催乳素在渗透压调节中起着重要作用,并且相互之间存在一定关联。 |
| 关键词: 银鲳 盐度 Na /K -ATP酶 渗透压调节 激素 |
| DOI： |
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| 基金项目:国家科技支撑项目(2011BAD13B01)；中央级公益性科研院所基本科研业务费项目(东2011M09号)资助. |
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| The effects of salinity on Na /K -ATPase activity and plasma osmoregulatory hormone concentration in silver pomfret (Pampus argenteus) |
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ZHANG Chen-Jie,PENG Shi-Ming,WANG Jian-Gang and SHI Zhao-Hong
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Key Laboratory of East China Sea Oceanic Fishery Resources Exploitation and Utilization,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea Oceanic Fishery Resources Exploitation and Utilization,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea Oceanic Fishery Resources Exploitation and Utilization,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea Oceanic Fishery Resources Exploitation and Utilization,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;China;College of fisheries and life,Shanghai Ocean University;China
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| Abstract: |
| The juvenile silver pomfret were cultured in salinity 14, 25, 36 for 96h. And their gill Na /K -ATPase (NKA) activity, plasma growth hormone, Insulin-like growth factor-Ⅰ(IGF-Ⅰ) and prolactin (PRL) concentrations were tested with different salinity and time in this experiment. The results show that gill NKA activities increased firstly then recovered as a whole; salinity 14 at 48h, the highest point, was significantly different from other points (P < 0.05).The plasma GH concentrations of salinity 36 which keeping high levels were significantly different from other salinity (P < 0.05), and point at 8h was the highest value. The plasma IGF-Ⅰ concentrations of salinity 14 and 36 both increased firstly then recovered. The plasma PRL concentrations of salinity 14 were always higher than other salinity groups, and its points at 8h, 24h and 48h were significantly higher (P < 0.05). This experiment also found that plasma GH concentration and gill NKA activity were significantly positively correlated (P < 0.01) in low salinity adaption (14 and 25); plasma PRL concentration and gill NKA activity were significantly negatively correlated (P < 0.01) in high salinity adaption (36 and 25). In addition, plasma PRL and IGF-Ⅰ concentrations were significantly positively correlated (P < 0.01). This research proved that gill NKA, plasma GH, PRL and IGF-Ⅰ were involved in osmoregulation of silver pomfret adapting for different salinity. |
| Key words: silver pomfret salinity Na /K -ATPase osmoregulation hormone |