| 摘要: |
| 以肌动球蛋白含量、Ca2+-ATPase酶活性、总巯基活性(T-SH)和活性巯基(A-SH)含量为考察指标, 研究了用不同pH的酸性电解水、以不同的时间对凡纳滨对虾进行减菌化处理后, 对虾肌肉的生理特性及肌肉品质的变化。结果表明: 电解水的酸性越强、处理时间越长, 对虾的肌动球蛋白、T-SH和A-SH的含量越低, Ca2+-ATPase的酶活越小。当对虾在pH分别为2.62、3.18、4.06的酸性电解水中处理60min后, 肌动球蛋白含量分别损失了98.7%、90.0%、73.7%, Ca2+-ATPase的失活率分别为93.4%、87.8%、84.3%, T-SH含量分别减少了57.8%、42.3%、40.3%, A-SH含量分别减少了77.8%、65.9%、30.7%。说明酸性电解水的减菌化条件对虾肉的生化指标影响显著。 |
| 关键词: 酸性电解水, 肌动球蛋白, Ca2+-ATPase, 总巯基, 活性巯基 |
| DOI：10.11693/hyhz201206034034 |
| 分类号: |
| 基金项目:浙江省重大科技计划项目(2012C33084)资助 |
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| EFFECT OF DIFFERENT PARAMETERS OF ACIDIC ELECTROLYZED WATER BACTERIA-INHIBITING ON THE PHYSIOLOGICAL PROPERTY OF LITOPENAEUS VANNAMEI |
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WU Dong-Mei, XIE Chao, LUO Hong-Yu
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School of Food Science and Pharmacy, Zhejiang Ocean University
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| Abstract: |
| Acidic electrolytic water was applied in this study to inhibit the bacteria from Litopenaeus vannamei. Several assessment standards of physiological property such as actomyosin content, Ca2+-ATPase enzyme activity, total sulfhydryl activity (T-SH) and active sulfhydryl (A-SH) content were measured to evaluate the influence of using acidic electrolytic water with different pH value or treating time on the meat quality of L. vannamei. The data obtained from the experiment showed that the actomyosin, T-SH, A-SH content and Ca2+-ATPase enzyme activity had a large decrease when using the acidic electrolytic water at a low pH value or extending the treatment time. The detail changes of each assessment standard after the treatment of acidic electrolytic water with the pH value 2.62, 3.18 and 4.06 for 60 minutes were respectively shown as follows: the content of actomyosin had a decrease of 98.7%, 90.0% and 73.7% and the inactive rates of Ca2+-ATPase were 93.4%, 87.8% and 84.3%. Results also showed the content of T-SH had a decrease of 57.8%, 42.3% and 40.3% and the content of A-SH had a decrease of 77.8%, 65.9%,30.7% as well, which indicated the conditions of using the acidic electrolytic water had a significant impact on the quality of L. vannamei. |
| Key words: Acidic electrolyzed water, Actomyosin, Ca2+-ATPase, Total sulphur, Active sulphur |
